College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2023 Apr 14;24(8):7245. doi: 10.3390/ijms24087245.
Under climate change, drought is one of the most limiting factors that influences wheat ( L.) production. Exploring stress-related genes is vital for wheat breeding. To identify genes related to the drought tolerance response, two common wheat cultivars, Zhengmai 366 (ZM366) and Chuanmai 42 (CM42), were selected based on their obvious difference in root length under 15% PEG-6000 treatment. The root length of the ZM366 cultivar was significantly longer than that of CM42. Stress-related genes were identified by RNA-seq in samples treated with 15% PEG-6000 for 7 days. In total, 11,083 differentially expressed genes (DEGs) and numerous single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) were identified. GO enrichment analysis revealed that the upregulated genes were mainly related to the response to water, acidic chemicals, oxygen-containing compounds, inorganic substances, and abiotic stimuli. Among the DEGs, the expression levels of 16 genes in ZM366 were higher than those in CM42 after the 15% PEG-6000 treatment based on RT-qPCR. Furthermore, EMS-induced mutants in Kronos ( L.) of 4 representative DEGs possessed longer roots than the WT after the 15% PEG-6000 treatment. Altogether, the drought stress genes identified in this study represent useful gene resources for wheat breeding.
在气候变化下，干旱是影响小麦（L.）生产的最主要限制因素之一。探索与胁迫相关的基因对于小麦的培育至关重要。为了鉴定与耐旱性响应相关的基因，根据在 15%聚乙二醇-6000 处理下根长的明显差异，选择了两个普通小麦品种郑麦 366（ZM366）和川麦 42（CM42）。ZM366 品种的根长明显长于 CM42。用 RNA-seq 鉴定在 15%PEG-6000 处理 7 天后的应激相关基因。共鉴定到 11083 个差异表达基因（DEGs）和大量单核苷酸多态性（SNP）和插入/缺失（InDel）。GO 富集分析表明，上调基因主要与对水、酸性化学物质、含氧化合物、无机物质和非生物刺激的响应有关。在 DEGs 中，根据 RT-qPCR，在 15%PEG-6000 处理后，ZM366 中 16 个基因的表达水平高于 CM42。此外，在 15%PEG-6000 处理后，4 个代表性 DEGs 的 Kronos（L.）的 EMS 诱导突变体的根长比 WT 长。总之，本研究中鉴定的耐旱性相关基因代表了小麦培育的有用基因资源。
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