生成用于不同肺毛细血管群体基因靶向的Plvap-CreER和Car4-CreER。
Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations.
作者信息
Zhang Zhenqian, Zhou Bin
机构信息
State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China.
State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
出版信息
J Genet Genomics. 2022 Dec;49(12):1093-1100. doi: 10.1016/j.jgg.2022.08.001. Epub 2022 Aug 23.
It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs: gCap (general capillary) and aCap (aerocyte). They are identified by two unique markers, respectively: plasmalemmal vesicle-associated protein (PLVAP) and carbonic anhydrase IV (CAR4). Here, we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER, which genetically target gCap and aCap, respectively. Induced by tamoxifen, the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP gCap and CAR4 aCap, respectively, in the lungs. These two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP and CAR4 cells in lung homeostasis, injury and repair.
最近有报道称，哺乳动物肺中存在两种不同的毛细血管内皮细胞亚群：gCap（普通毛细血管）和aCap（气细胞）。它们分别由两种独特的标志物鉴定：质膜囊泡相关蛋白（PLVAP）和碳酸酐酶IV（CAR4）。在此，我们报告了两种新型的敲入小鼠品系Plvap-CreER和Car4-CreER，它们分别在基因上靶向gCap和aCap。在他莫昔芬的诱导下，Plvap-CreER和Car4-CreER等位基因分别在肺中的PLVAP gCap和CAR4 aCap中介导特异性和高效的Cre-loxP重组。这两种小鼠品系是研究PLVAP和CAR4细胞在肺稳态、损伤和修复中的细胞命运和功能的有用遗传工具。
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