Ginsburg-Moraff Carol, Grob Jonathan, Chin Karl, Eastman Grant, Wildhaber Sandra, Bayliss Mark, Mues Heinrich M, Palmieri Marco, Poirier Jennifer, Reck Marcel, Luneau Alexandre, Rodde Stephane, Reilly John, Wagner Trixie, Brocklehurst Cara E, Wyler René, Dunstan David, Marziale Alexander N
Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
SLAS Technol. 2022 Dec;27(6):350-360. doi: 10.1016/j.slast.2022.08.002. Epub 2022 Aug 24.
We herein report the development of an automation platform for rapid purification and quantification of chemical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on analytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.
我们在此报告了一个自动化平台的开发，该平台用于化学文库的快速纯化和定量，包括将化学物质重新配制为10 mM的二甲基亚砜储备溶液。这个完全集成的工作流程基于分析数据，具有针对微尺度制备型反相（RP）HPLC-MS的定制条件、在线馏分质量控制和基于CAD的定量，以及自动化重新格式化，以实现化学文库的快速纯化。这个自动化工作流程完全基于溶液，无需称量或处理固体。这提高了过程效率，并在高通量合成与新型化学物质在相关测定中的生物学和物理化学性质分析之间建立了联系。
