IRD, AP-HM, SSA, VITROME, Aix-Marseille University, Marseille 13005, France.
Faculty of Sciences Ben M'sik, Laboratory of Biology and Health, Hassan II university of Casablanca, Morocco 20670, Morocco.
FEMS Microbiol Lett. 2022 Sep 20;369(1). doi: 10.1093/femsle/fnac066.
Strain Marseille-P4119T was isolated from a faecal sample of a healthy 32-year-old faecal transplant donor. The bacterium was anaerobic, Gram-negative, rod-shaped, non-motile, and did not produce spores. We studied its phenotypic characteristics and sequenced its whole genome. The major fatty acids were C15:0anteiso and C15:0iso. The final genome assembly was 3912650 bp long with a 44.4 mol% G + C content, 3094 protein-coding genes and 74 RNA genes. Strain Marseille-P4119T exhibited a 97.10% 16S rRNA sequence identity and a 29.0% dDDH with Prevotella stercorea CB35T, OrthoANI values ranged from 68.5% with Prevotella enoeca to 77.4% with Prevotella stercorea, the phylogenetically closest bacterial species with standing in nomenclature. Based on the phylogenetic, phenotypic and genomic analyses, we propose the creation of the novel species Prevotella merdae sp. nov. The type strain is Marseille-P4119T ( = CSUR P4119T = CECT 9566T).
马赛 P4119T 株从一位 32 岁健康粪便移植供体的粪便样本中分离得到。该细菌为厌氧菌,革兰氏阴性,杆状,不运动,不产生孢子。我们研究了其表型特征并对其全基因组进行了测序。主要脂肪酸为 C15:0anteiso 和 C15:0iso。最终基因组组装长度为 3912650bp,G+C 含量为 44.4mol%,包含 3094 个蛋白编码基因和 74 个 RNA 基因。马赛 P4119T 株 16S rRNA 序列的同一性为 97.10%,与 Prevotella stercorea CB35T 的 dDDH 为 29.0%,与 Prevotella enoeca 的 OrthoANI 值范围为 68.5%至 77.4%,与 Prevotella stercorea 最为接近,后者是具有命名地位的系统发育上最接近的细菌种。基于系统发育、表型和基因组分析,我们建议创建新的 Prevotella merdae 种。模式菌株为马赛 P4119T(=CSUR P4119T=CECT 9566T)。