Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, University of Naples Federico II, 80131 Naples, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "L. Vanvitelli", 81100, Caserta, Italy.
Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, University of Naples Federico II, 80131 Naples, Italy.
Biomed Pharmacother. 2022 Oct;154:113587. doi: 10.1016/j.biopha.2022.113587. Epub 2022 Aug 25.
The limited therapeutic options for ischemic stroke treatment render necessary the identification of new strategies. In recent years, it has been shown that natural compounds may represent a valid therapeutic opportunity. Therefore, the present study aimed to evaluate the protective effect of Ruta graveolens water extract (RGWE) in an in vivo experimental model of brain ischemia.
RGWE effects on ischemic damage and neurological function were evaluated in adult rats subjected to transient occlusion of the Middle Cerebral Artery (tMCAO), receiving two intraperitoneal injections of RGWE, 100 and 300 min after the induction of ischemia. In addition, astroglial and microglial activation was measured as GFAP and IBA-1 expression by immunofluorescence and confocal microscopy analysis.
Treatment with RGWE containing 10 mg/kg of Rutin, the major component, ameliorates the ischemic damage and improves neurological performances. Interestingly, the pro-inflammatory states of astrocytes and microglia, respectively detected by using C3 and iNOS markers, were significantly reduced in ipsilateral cortical and striatal areas in ischemic RGWE-treated rats.
RGWE shows a neuroprotective effect on brain infarct volume extent in a transient focal cerebral ischemia model and this effect was paralleled by the prevention of pro-inflammatory astroglial and microglial activation. Collectively, our findings support the idea that natural compounds may represent potential therapeutic opportunities against ischemic stroke.
缺血性中风治疗的有限治疗选择使得有必要寻找新的策略。近年来，已经表明天然化合物可能代表一种有效的治疗机会。因此，本研究旨在评估芫荽水提取物（RGWE）在体内脑缺血实验模型中的保护作用。
将 RGWE 作用于接受短暂性大脑中动脉闭塞（tMCAO）的成年大鼠，在缺血诱导后 100 和 300 分钟进行两次腹腔注射，评估 RGWE 对缺血性损伤和神经功能的影响。此外，通过免疫荧光和共聚焦显微镜分析测量星形胶质细胞和小胶质细胞的激活，以 GFAP 和 IBA-1 的表达来衡量。
含有 10 毫克/千克芦丁（主要成分）的 RGWE 治疗可改善缺血性损伤并改善神经功能。有趣的是，在缺血性 RGWE 治疗大鼠的同侧皮质和纹状体区域，用 C3 和 iNOS 标志物检测到的星形胶质细胞和小胶质细胞的促炎状态显著降低。
RGWE 在短暂性局灶性脑缺血模型中显示出对脑梗死体积的神经保护作用，这种作用与预防促炎星形胶质细胞和小胶质细胞激活平行。总之，我们的发现支持这样一种观点，即天然化合物可能代表针对缺血性中风的潜在治疗机会。
