Department of Laboratory Medicine, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai 200003, China.
Department of Laboratory Medicine, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai 200003, China.
Int Immunopharmacol. 2022 Oct;111:109155. doi: 10.1016/j.intimp.2022.109155. Epub 2022 Aug 24.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease involving multiple system functions. Our study aimed to screen out more effective new indicators that can assist clinical diagnosis and judge disease activity.
We first screened serum levels of 45 cytokines of SLE patients (n = 3) and healthy controls (n = 3). Subsequently, we selected five elevated cytokines for verification with an expanded sample size. Then, the relationship between cytokines and laboratory parameters was also investigated. Finally, we used receiver operating characteristic (ROC) curves to assess the clinical value of these cytokines.
Through screening of 45 cytokines, 15 were found to be elevated in SLE patients. We chose five cytokines (IL-6, IL-10, IL-1RA, IP-10 and LIF) for further research and found elevated expression of all five cytokines in SLE patients. Serum levels of IL-10, IL-1RA and LIF were positively correlated with SLEDAI-2K score. Besides, the level of IL-10 was significantly positively correlated with serum IgG and erythrocyte sedimentation rate (ESR); IL-1RA was significantly negatively correlated with C3 and C4; and LIF was significantly positively correlated with serum IgG, C-reactive protein (CRP), and ESR. Furthermore, IL-1RA and LIF were strongly positively correlated with 24-hour urine protein levels. The ROC analysis showed that IL-1RA has good diagnostic value, and IL-10 and LIF levels can be utilized to discriminate between active and inactive SLE.
IL-1RA can be used as a biomarker for diagnosing SLE, while IL-10 and LIF can be indicators to discriminate between active and inactive SLE.
系统性红斑狼疮（SLE）是一种涉及多个系统功能的慢性自身免疫性疾病。我们的研究旨在筛选出更有效的新指标，以协助临床诊断和判断疾病活动度。
我们首先筛选了 3 例 SLE 患者和 3 例健康对照者的血清 45 种细胞因子水平。随后，我们选择了五种升高的细胞因子进行扩大样本量验证。然后，还研究了细胞因子与实验室参数之间的关系。最后，我们使用接收者操作特征（ROC）曲线来评估这些细胞因子的临床价值。
通过筛选 45 种细胞因子，发现 15 种细胞因子在 SLE 患者中升高。我们选择了五种细胞因子（IL-6、IL-10、IL-1RA、IP-10 和 LIF）进行进一步研究，发现所有五种细胞因子在 SLE 患者中均有升高表达。血清 IL-10、IL-1RA 和 LIF 水平与 SLEDAI-2K 评分呈正相关。此外，IL-10 水平与血清 IgG 和红细胞沉降率（ESR）显著正相关；IL-1RA 与 C3 和 C4 显著负相关；LIF 与血清 IgG、C 反应蛋白（CRP）和 ESR 显著正相关。此外，IL-1RA 和 LIF 与 24 小时尿蛋白水平呈强烈正相关。ROC 分析表明，IL-1RA 具有良好的诊断价值，而 IL-10 和 LIF 水平可用于区分 SLE 的活动期和非活动期。
IL-1RA 可作为诊断 SLE 的生物标志物，而 IL-10 和 LIF 可作为区分活动期和非活动期 SLE 的指标。
