Zhu Qiuyu, Cui Jiuming, Liu Sen, Wei Suosu, Wu Qiuxia, You Yanwu
Department of Nephrology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
Department of Scientific Cooperation, Guangxi Academy of Medical Sciences, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
Front Microbiol. 2024 Apr 2;15:1295378. doi: 10.3389/fmicb.2024.1295378. eCollection 2024.
To investigate the changes in gut microbes and their metabolites after administering synbiotics to patients with new-onset lupus nephritis (LN) treated using a conventional method and provide a theoretical basis for finding new targets for the diagnosis and treatment of LN.
In this study, a total of 12 participants were divided into the lupus and synbiotic groups. Stool samples and clinical data were collected before and after treatment for metagenomic, nontargeted metabolomic, and statistical analyses.
The relative abundances of the pathogenic bacteria and Enterobacteriaceae_unclassified decreased after synbiotic treatment, whereas the abundances of Actinobacteria and Firmicutes increased. Further, the Firmicutes to Bacteroidetes ratio increased; however, the difference was not statistically significant ( > 0.05). α diversity analysis showed no significant differences in the intestinal microbial richness and diversity index of patients with LN between the groups before and after treatment ( > 0.05). β analysis showed the differences in the community structure between the samples of the two groups before and after treatment. Linear discriminant analysis effect size and receiver operating characteristic curve analyses revealed that Negativicutes (AUC = 0.9722) and Enterobacteriaceae_unclassified (AUC = 0.9722) were the best predictors of the lupus and synbiotic groups, respectively, before and after treatment. Joint analyses revealed that amino acid biosynthesis, aminoacyl-tRNA biosynthesis, purine metabolism, and other metabolic pathways may be involved in the changes in the metabolic function of patients with LN after the addition of synbiotics. Spearman's correlation analysis revealed the interaction between clinical features and flora, and flora exhibited a complex biological network regulatory relationship.
Synbiotics regulate the metabolic functions of intestinal microorganisms in patients with LN and play a role in various biological functions. Synbiotic supplements may be safe and promising candidates for patients with LN.
研究新型狼疮性肾炎（LN）患者采用传统方法治疗后服用合生元后肠道微生物及其代谢产物的变化，为寻找LN诊断和治疗的新靶点提供理论依据。
本研究共纳入12名参与者，分为狼疮组和合生元组。在治疗前后收集粪便样本和临床数据，进行宏基因组学、非靶向代谢组学和统计分析。
合生元治疗后，病原菌和未分类肠杆菌科的相对丰度降低，而放线菌和厚壁菌的丰度增加。此外，厚壁菌与拟杆菌的比例增加；然而，差异无统计学意义（>0.05）。α多样性分析显示，LN患者治疗前后组间肠道微生物丰富度和多样性指数无显著差异（>0.05）。β分析显示两组治疗前后样本的群落结构存在差异。线性判别分析效应大小和受试者工作特征曲线分析显示，Negativicutes（AUC = 0.9722）和未分类肠杆菌科（AUC = 0.9722）分别是狼疮组和合生元组治疗前后的最佳预测指标。联合分析显示，氨基酸生物合成、氨酰-tRNA生物合成、嘌呤代谢等代谢途径可能参与了LN患者添加合生元后代谢功能的变化。Spearman相关性分析揭示了临床特征与菌群之间的相互作用，菌群呈现出复杂的生物网络调节关系。
合生元调节LN患者肠道微生物的代谢功能，并在多种生物学功能中发挥作用。合生元补充剂可能是LN患者安全且有前景的候选药物。
