ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, Maharashtra 413115, India.
ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, Maharashtra 413115, India.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Nov;261:109447. doi: 10.1016/j.cbpc.2022.109447. Epub 2022 Aug 24.
The aquatic ecosystem is prone to global climate change and pollution affecting aquatic animals, including fish. In light of the above, we experimented with delineate the role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with selenium nanoparticles (Se-NPs) to enhance the thermal tolerance in Pangasianodon hypophthalmus reared under control or concurrent exposure to high temperature and arsenic (As + T) for 112 days. Se-NPs were synthesized using the green approach. Four experimental diets viz. EPA + DHA at 0.2, 0.4 and 0.6 % along with Se-NPs at 0.2 mg kg diet were formulated and prepared. End of the experiment (112 days), the thermal tolerance viz. CTmin (critical thermal minima) CTmax (critical thermal maxima), LTmin (lethal thermal minima) and LTmax (lethal thermal maxima) were determined. Supplementation of EPA + DHA along with Se-NPs noticeably improved the thermal tolerance of the fish reared under stress (As + T) and control condition. Superoxide dismutase, glutathione-s-transferase, catalase, glutathione peroxides and LPO were enhanced by As + T, whereas EPA + DHA at 0.4 % and Se-NPs reduced the oxidative stress. Further, acetylcholine esterase was inhibited by arsenic alone and concurrent with temperature but dietary supplementation significantly enhanced the brain AChE activity. Exposure to arsenic and concurrent with a temperature significantly reduced the ATPase. Whereas supplementation of EPA + DHA at 0.4 % and Se-NPs enhanced the ATPase in liver and gill tissues. Arsenic bioaccumulation was also reduced with EPA + DHA at 0.4 % and Se-NPs. The present investigation concluded that EPA + DHA at 0.4 % and Se-NPs at 0.2 mg kg diet protects the P. hypophthalmus against arsenic pollution and thermal stress.
水生生态系统容易受到全球气候变化和污染的影响，这些变化和污染会影响水生动物，包括鱼类。有鉴于此，我们实验了用二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）与硒纳米颗粒（Se-NPs）来增强在控制或同时暴露于高温和砷（As+T）条件下饲养的 Pangasianodon hypophthalmus 的耐热性，实验共进行了 112 天。Se-NPs 是通过绿色方法合成的。设计了四种实验饲料，即 0.2%、0.4%和 0.6%的 EPA+DHA 以及 0.2mg/kg 饲料的 Se-NPs。实验结束时（112 天），测定了耐热性，包括 CTmin（临界热最小值）、CTmax（临界热最大值）、LTmin（致死热最小值）和 LTmax（致死热最大值）。在应激（As+T）和对照条件下饲养的鱼类补充 EPA+DHA 与 Se-NPs 显著提高了其耐热性。超氧化物歧化酶、谷胱甘肽-S-转移酶、过氧化氢酶、谷胱甘肽过氧化物酶和 LPO 均因 As+T 而增加，而 0.4%的 EPA+DHA 和 Se-NPs 则降低了氧化应激。此外，砷单独和与温度同时暴露会抑制乙酰胆碱酯酶，但膳食补充显著增强了大脑 AChE 活性。砷暴露和同时暴露于高温会显著降低 ATPase。而补充 0.4%的 EPA+DHA 和 Se-NPs 则会增强肝脏和鳃组织中的 ATPase。EPA+DHA 与 Se-NPs 的添加也降低了砷的生物累积。本研究得出的结论是，0.4%的 EPA+DHA 和 0.2mg/kg 饲料的 Se-NPs 可保护 P. hypophthalmus 免受砷污染和热应激的影响。
