Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Organic Device Development Department, Material Development Division, Toyoda Gosei Co., Ltd., Ama 490-1207, Japan.
ACS Sens. 2024 Aug 23;9(8):4248-4255. doi: 10.1021/acssensors.4c01230. Epub 2024 Jul 30.
Microphysiological systems have attracted attention because of their use in drug screening. However, it is challenging to measure cell functions in real time using a device. In this study, we developed a cell culture device using a porous membrane electrode for in situ electrochemical glucose measurements for cell analysis. First, a porous membrane electrode was fabricated and electrochemically evaluated for enzyme-free glucose measurement. Subsequently, the glucose uptake of MCF-7 spheroids was evaluated using living spheroids, fixed spheroids, supernatants, and glucose transporter inhibitor-treated spheroids. Conventionally, the direct optical measurement of glucose uptake requires fluorescence-labeled glucose derivatives. In addition, the glucose uptake can be evaluated by measuring the glucose concentration in the medium by optical or electrochemical measurements. However, glucose needs to be consumed in the entire cell culture medium, which needs a long culture time. In contrast, our system can measure glucose in approximately 5 min without any labels because of in situ electrochemical measurements. This system can be used for in situ measurements in in vitro cell culture systems, including organ-on-a-chip for drug screening.
微生理系统因其在药物筛选中的应用而受到关注。然而，使用设备实时测量细胞功能具有挑战性。在这项研究中，我们开发了一种使用多孔膜电极的细胞培养装置，用于细胞分析的原位电化学葡萄糖测量。首先，制备了多孔膜电极，并对其进行了无酶葡萄糖测量的电化学评估。随后，使用活球体、固定球体、上清液和葡萄糖转运蛋白抑制剂处理的球体评估了 MCF-7 球体的葡萄糖摄取。传统上，葡萄糖摄取的直接光学测量需要荧光标记的葡萄糖衍生物。此外，还可以通过光学或电化学测量来测量培养基中的葡萄糖浓度来评估葡萄糖摄取。然而，由于需要在整个细胞培养基中消耗葡萄糖，因此需要较长的培养时间。相比之下，我们的系统可以在大约 5 分钟内进行无任何标记的葡萄糖测量，因为这是原位电化学测量的结果。该系统可用于药物筛选的器官芯片等体外细胞培养系统中的原位测量。
