College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Genes (Basel). 2024 May 6;15(5):589. doi: 10.3390/genes15050589.
Phosphorus (P) is a vital nutrient element that is essential for plant growth and development, and arbuscular mycorrhizal fungi (AMF) can significantly enhance P absorption. The phosphate transporter protein 1 (PHT1) family mediates the uptake of P in plants. However, the gene has not yet been characterized in . In this study, to gain insight into the functional divergence of genes, nine genes were identified in the genome database via bioinformatics tools. Phylogenetic analysis revealed that the PHT1 proteins of , , and could be divided into three groups. PHT1 in the same clade has a similar gene structure and motif, suggesting that the features of each clade are relatively conserved. Further tissue expression analysis revealed that was expressed mainly in the roots and stems. In addition, phenotypic changes, P content, and gene expression were analyzed in plants inoculated with AMF under different P conditions (0 mM, 0.1 mM, and 10 mM). P stress and AMF significantly affected the growth and P accumulation of . was strongly expressed in the roots colonized by AMF, implying that was a specific AMF-inducible PHT1. Taken together, these results provide new insights into the functional divergence and genetic redundancy of the genes in response to P stress and AMF symbiosis in .
磷（P）是植物生长和发育所必需的重要营养元素，丛枝菌根真菌（AMF）可以显著增强 P 的吸收。磷酸转运蛋白 1（PHT1）家族介导植物中 P 的摄取。然而，该基因在 中尚未得到表征。在这项研究中，为了深入了解 基因的功能分化，通过生物信息学工具在 基因组数据库中鉴定出 9 个 基因。系统发育分析表明，来自 、 和 的 PHT1 蛋白可以分为三组。同一进化枝中的 PHT1 具有相似的基因结构和基序，这表明每个进化枝的特征相对保守。进一步的组织表达分析表明， 主要在根和茎中表达。此外，在不同 P 条件（0 mM、0.1 mM 和 10 mM）下，分析了接种 AMF 的 植物的表型变化、P 含量和 基因表达。P 胁迫和 AMF 显著影响 的生长和 P 积累。 在被 AMF 定殖的根中强烈表达，这表明 是一种特定的 AMF 诱导型 PHT1。综上所述，这些结果为 基因在应对 P 胁迫和 AMF 共生中的功能分化和遗传冗余提供了新的见解。
