Key Laboratory of Clinical diagnosis and treatment of Animal Diseases, Department of Agriculture and villages, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, Hohhot 010018, China.
Key Laboratory of Clinical diagnosis and treatment of Animal Diseases, Department of Agriculture and villages, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, Hohhot 010018, China.
Virus Res. 2022 Oct 15;320:198899. doi: 10.1016/j.virusres.2022.198899. Epub 2022 Aug 27.
Infectious bovine rhinotracheitis (IBR) is caused by Bovine herpesvirus type 1 (BoHV-1), which seriously threatens the global cattle industry. Only vaccination to improve immunity is the most direct and effective means to prevent IBR. Attempts are being made to use subunit vaccines, deleted or recombinant viral vaccines to reduce or eradicate IBR. For investigating the immunological characteristics of glycoprotein B subunit vaccine in pattern animal guinea pigs, the partial glycoprotein B (gB) of BoHV-1 with dominant antigenic characteristic was selected. A recombinant prokaryotic expression vector pET-32a-gB with the truncated gB gene was constructed, expressed, identified and the purified proteins were used to immunize guinea pigs. The immune effect of the subunit vaccine was assessed by monitoring clinical symptoms, viral load, antibody secretion, and histopathological changes. The results indicated that guinea pigs immunized with the gB subunit vaccine produced high levels of anti-gB antibodies and virus-neutralizing antibodies. The gB subunit vaccine significantly reduced viral shedding and lung tissue damage after IBRV challenge. The animals inoculated the gB subunit vaccine also had less virus reactivation. Its protective effect on viral shedding and tissue damage was similar to that of inactivated BoHV-1 vaccine. This work is a proof-of-concept study of subunit vaccine-induced protection against BoHV-1. And it is expected to be a candidate vaccine for the prevention of IBR.
传染性牛鼻气管炎（IBR）由牛疱疹病毒 1 型（BoHV-1）引起，严重威胁着全球牛养殖业。只有通过接种疫苗来提高免疫力，才是预防 IBR 的最直接、最有效的手段。目前，人们正在尝试使用亚单位疫苗、缺失或重组病毒疫苗来减少或根除 IBR。为了研究 BoHV-1 糖蛋白 B 亚单位疫苗在模式动物豚鼠中的免疫特性，选择具有优势抗原特性的 BoHV-1 糖蛋白 B （gB）的部分片段。构建了带有截断 gB 基因的重组原核表达载体 pET-32a-gB，并进行了表达、鉴定，纯化后的蛋白被用来免疫豚鼠。通过监测临床症状、病毒载量、抗体分泌和组织病理学变化，评估了亚单位疫苗的免疫效果。结果表明，gB 亚单位疫苗免疫的豚鼠产生了高水平的抗-gB 抗体和病毒中和抗体。在 IBRV 攻毒后，gB 亚单位疫苗显著减少了病毒脱落和肺组织损伤。接种 gB 亚单位疫苗的动物病毒再激活也较少。其对病毒脱落和组织损伤的保护作用与灭活 BoHV-1 疫苗相似。这项工作是针对 BoHV-1 亚单位疫苗诱导保护作用的概念验证研究，有望成为预防 IBR 的候选疫苗。
