College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Viruses. 2022 Jul 25;14(8):1618. doi: 10.3390/v14081618.
Bovine respiratory disease complex (BRDC) is a comprehensive disease in cattle caused by various viral and bacterial infections. Among them, bovine herpesvirus type I (BoHV-1) and bovine viral diarrhea virus (BVDV) play important roles and have caused huge financial losses for the cattle industry worldwide. At present, vaccines against BRDC include trivalent attenuated BoHV-1, BVDV-1, and BVDV-2 live vaccines, BoHV-1 live attenuated vaccines, and BoHV-1/BVDV bivalent live attenuated vaccines, which have limitations in terms of their safety and efficacy. To solve these problems, we optimized the codon of the BVDV-1 E2 gene, added the signal peptide sequence of the BoHV-1 gD gene, expressed double BVDV-1 E2 glycoproteins in tandem at the BoHV-1 gE gene site, and constructed a BoHV-1 genetics-engineered vectored vaccine with gE gene deletion, named BoHV-1 gE/E2-Linker-E2 and BoHV-1 ΔgE. This study compared the protective effects in BoHV-1, BoHV-1 ΔgE, BoHV-1 gE/E2-Linker-E2, and BVDV-1 inactivated antigen immunized guinea pigs and calves. The results showed that BoHV-1 gE/E2-Linker-E2 could successfully induce guinea pigs and calves to produce specific neutralizing antibodies against BVDV-1. In addition, after BoHV-1 and BVDV-1 challenges, BoHV-1 gE/E2-Linker-E2 can produce a specific neutralizing antibody response against BoHV-1 and BVDV-1 infections. Calves immunized with this type of virus can be distinguished as either vaccinated animals (gE) or naturally infected animals (gE). In summary, our data suggest that BoHV-1 gE/E2-Linker-E2 and BoHV-1 ΔgE have great potential to prevent BVDV-1 or BoHV-1 infection.
牛呼吸道疾病复合症（BRDC）是一种由多种病毒和细菌感染引起的牛综合性疾病。其中，牛疱疹病毒 1 型（BoHV-1）和牛病毒性腹泻病毒（BVDV）起着重要作用，给全球牛养殖业造成了巨大的经济损失。目前，BRDC 疫苗包括三价弱毒 BoHV-1、BVDV-1 和 BVDV-2 活疫苗、BoHV-1 活弱毒疫苗和 BoHV-1/BVDV 二价活弱毒疫苗，但这些疫苗在安全性和有效性方面存在局限性。为了解决这些问题，我们优化了 BVDV-1 E2 基因的密码子，添加了 BoHV-1 gD 基因的信号肽序列，在 BoHV-1 gE 基因位点串联表达双 BVDV-1 E2 糖蛋白，构建了 BoHV-1 基因工程载体疫苗 gE 基因缺失株，命名为 BoHV-1 gE/E2-Linker-E2 和 BoHV-1ΔgE。本研究比较了 BoHV-1、BoHV-1ΔgE、BoHV-1 gE/E2-Linker-E2 和 BVDV-1 灭活抗原免疫豚鼠和犊牛的保护效果。结果表明，BoHV-1 gE/E2-Linker-E2 能成功诱导豚鼠和犊牛产生针对 BVDV-1 的特异性中和抗体。此外，在 BoHV-1 和 BVDV-1 攻毒后，BoHV-1 gE/E2-Linker-E2 能产生针对 BoHV-1 和 BVDV-1 感染的特异性中和抗体反应。用该病毒免疫的犊牛可分为接种动物（gE）或自然感染动物（gE）。综上所述，我们的数据表明，BoHV-1 gE/E2-Linker-E2 和 BoHV-1ΔgE 具有预防 BVDV-1 或 BoHV-1 感染的巨大潜力。
