Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China.
Xiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, 261312, China.
Fish Shellfish Immunol. 2022 Oct;129:251-262. doi: 10.1016/j.fsi.2022.08.061. Epub 2022 Aug 27.
Penaeus japonicas is an important shrimp species, which is exposed to stressors including a variety of epidemic diseases. To date, little is known about the mechanisms involved in the response to white spot syndrome virus (WSSV) mediated by long non-coding RNAs (lncRNAs). A total of 6544 putative lncRNAs were identified in the hepatopancreas in P. japonicas, which provides a useful lncRNA reference resource for use in future studies. In addition, a total of 444 differentially expressed mRNAs and 457 differentially expressed lncRNAs were identified at 6, 12, and 24 h after WSSV infection in the hepatopancreas of P. japonicas. Functional enrichment analysis showed that the differentially expressed mRNAs were enriched in terms related to immune response and viral infectivity such as defense response, aminopeptidase activity, whereas the differentially expressed lncRNA partner genes were enriched in ubiquitin-dependent protein catabolic process, lipoprotein metabolic process, and antigen processing and presentation. Moreover, several lncRNAs were induced by WSSV infection, indicating these lncRNAs might participate in regulating many immune processes referring to their partner genes. Co-expression analysis of the lncRNAs and their partner genes identified some high lncRNA-mRNA correlations. These results suggest that WSSV stimulates the immune response in the hepatopancreas potentially through an important coding and non-coding gene network, thereby providing valuable information regarding non-coding responses to WSSV in Penaeus species.
日本对虾是一种重要的虾类，易受到各种流行疾病等应激源的影响。迄今为止，人们对长链非编码 RNA（lncRNA）介导的对白斑综合征病毒（WSSV）的反应机制知之甚少。本研究共鉴定出日本对虾肝胰腺中的 6544 个推定的 lncRNA，为今后的研究提供了有用的 lncRNA 参考资源。此外，在日本对虾肝胰腺中，WSSV 感染后 6、12 和 24 h 分别鉴定到 444 个差异表达的 mRNAs 和 457 个差异表达的 lncRNAs。功能富集分析表明，差异表达的 mRNAs 富集在与免疫反应和病毒感染力相关的术语中，如防御反应、氨肽酶活性，而差异表达的 lncRNA 伙伴基因富集在泛素依赖性蛋白分解代谢过程、脂蛋白代谢过程和抗原加工和呈递中。此外，一些 lncRNAs 被 WSSV 感染诱导，表明这些 lncRNAs 可能通过其伙伴基因参与调节许多免疫过程。lncRNA 和其伙伴基因的共表达分析确定了一些高 lncRNA-mRNA 相关性。这些结果表明，WSSV 可能通过重要的编码和非编码基因网络刺激肝胰腺中的免疫反应，从而为对虾属中 WSSV 的非编码反应提供有价值的信息。
