Universidade Federal do Rio Grande - FURG, Rua Visconde de Paranaguá, 102, Centro, Rio Grande, RS, 96203-900, Brazil.
Universidade Federal do Rio Grande - FURG, Rua Visconde de Paranaguá, 102, Centro, Rio Grande, RS, 96203-900, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2022 Sep;881:503521. doi: 10.1016/j.mrgentox.2022.503521. Epub 2022 Jun 30.
Growing evidence suggests that early-life events can predispose the newborn to a variety of health problems in postnatal life, which can lead to the need for specialized care in the neonatal intensive care unit (NICU). These events may be caused by factors intrinsically related to the mother (i.e., lifestyle, socioeconomic conditions), and this interplay between maternal exposure factors and negative outcomes in the neonate can be efficiently monitored through effect biomarkers, such as DNA damage. Thus, the present study aimed to evaluate the DNA damage and the maternal and neonatal factors associated with the genotoxic outcome using newborns admitted to the NICUs of three hospitals located in the extreme south of Brazil. A total of 81 newborns were evaluated. DNA damage was assessed using the comet assay, and according to the result obtained for the evaluated parameters (tail length, % of tail DNA and tail moment). The investigation of associated factors was performed using the bivariate and multivariate Poisson regression analysis. As a result, we observed that the tail moment was the most sensitive parameter to detect differences between variables and genetic outcomes in newborns from NICU. Birthweight and the presence of respiratory diseases were associated with greater risks of DNA damage. Furthermore, the variables family income, sex, head circumference, preterm, birthweight and the presence of respiratory and/or infectious diseases showed a significant statistical difference regarding the groups with and without DNA damage (based on the median of the parameter). While the results of this study will serve as the basis for investigating genetic damage, we encourage that similar studies should be conducted elsewhere in order to confirm these and other outcomes as associated factors with DNA damage in newborns.
越来越多的证据表明，生命早期的事件可能使新生儿在出生后容易出现各种健康问题，这可能导致他们需要在新生儿重症监护病房（NICU）接受特殊护理。这些事件可能是由与母亲内在相关的因素（即生活方式、社会经济条件）引起的，而母亲暴露因素与新生儿负面结果之间的这种相互作用可以通过有效的生物标志物（如 DNA 损伤）进行监测。因此，本研究旨在评估使用来自巴西南部三家医院 NICU 收治的新生儿评估 DNA 损伤以及与遗传毒性结果相关的母体和新生儿因素。共评估了 81 名新生儿。使用彗星试验评估 DNA 损伤，并根据评估参数（尾部长度、尾部 DNA 的百分比和尾部矩）的结果。使用双变量和多变量泊松回归分析进行相关因素的调查。结果表明，在 NICU 新生儿中，尾部矩是检测变量和遗传结果之间差异的最敏感参数。出生体重和呼吸道疾病的存在与更大的 DNA 损伤风险相关。此外，家庭收入、性别、头围、早产、出生体重以及呼吸道和/或传染病的存在等变量在 DNA 损伤组和无 DNA 损伤组之间存在显著的统计学差异（基于参数中位数）。虽然这项研究的结果将为遗传损伤的研究提供基础，但我们鼓励在其他地方进行类似的研究，以确认这些和其他结果作为与新生儿 DNA 损伤相关的因素。
