Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Veterinary Diagnostic Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
J Vet Med Sci. 2022 Oct 1;84(10):1377-1384. doi: 10.1292/jvms.22-0276. Epub 2022 Aug 26.
The carbapenemase-producing Acinetobacter baumannii is an important opportunistic bacterium and frequently causes hospital-acquired infections in humans. It also has increasingly been reported in veterinary medicine. This study illustrates multiple clones of carbapenemase-producing A. baumannii disseminating and causing diseases in dogs and cats in Thailand. Between 2016 and 2020, 44 A. baumannii and two A. pittii isolates exhibiting imipenem resistance (MIC≥16 μg/mL) from diagnostic samples were characterized by Pasteur multilocus sequence typing (MLST), sequence grouping (SG), repetitive extragenic palindromic element (rep)-PCR fingerprint analysis and antimicrobial resistance (AMR) profiling. All isolates contained bla in the Tn2006 family, and A. baumannii showed the sequence type (ST) 16 (14/44), ST149 (12/44), ST25 (6/44), ST2 (4/44), ST1581 (3/44), ST23 (2/44), ST1575 (1/44) and ST1576 (1/44). DNA fingerprint analysis and SG illustrated clonal relationships in the STs and its single locus variants, and AMR gene profiles, including tetracycline and aminoglycoside resistance genes, showed minor variations in the clones. The findings suggest that bla has been spread in multiple clones of A. baumannii and A. pittii from canine and feline hosts. With the collection of multiple AMR genes and intrinsic resistance, antimicrobial options are limited for treatment, and pets can be a potential reservoir of extensively drug-resistant, carbapenemase-producing A. baumannii in the community. Epidemiological tracking by passive and active surveillance in animals, veterinary personnel and hospital environment and preventive measurements should be promoted to decrease the risk of infection and transmission to humans.
产碳青霉烯酶鲍曼不动杆菌是一种重要的机会致病菌，经常导致人类医院获得性感染。它在兽医领域也越来越多地被报道。本研究说明了多个产碳青霉烯酶鲍曼不动杆菌克隆在泰国的犬和猫中传播并引起疾病。在 2016 年至 2020 年间，从诊断样本中分离出 44 株和 2 株对亚胺培南耐药（MIC≥16μg/mL）的鲍曼不动杆菌和 2 株鲍曼不动杆菌，采用巴斯德多位点序列分型（MLST）、序列群（SG）、重复外回文元件（rep）-PCR 指纹分析和抗菌药物耐药性（AMR）谱进行了特征分析。所有分离株均含有 Tn2006 家族的 bla，鲍曼不动杆菌显示序列型（ST）16（14/44）、ST149（12/44）、ST25（6/44）、ST2（4/44）、ST1581（3/44）、ST23（2/44）、ST1575（1/44）和 ST1576（1/44）。DNA 指纹分析和 SG 表明了 ST 及其单基因变异的克隆关系，而 AMR 基因谱，包括四环素和氨基糖苷类耐药基因，在克隆中显示出微小的变化。这些发现表明，bla 已经在来自犬和猫宿主的多个鲍曼不动杆菌和鲍曼不动杆菌克隆中传播。由于收集了多种 AMR 基因和固有耐药性，治疗的抗菌药物选择有限，宠物可能是社区中广泛耐药、产碳青霉烯酶鲍曼不动杆菌的潜在储库。应通过在动物、兽医人员和医院环境中进行被动和主动监测来进行流行病学跟踪，并采取预防措施，以降低感染和传播给人类的风险。
