Püntener-Simmen Sabrina, Zurfluh Katrin, Schmitt Sarah, Stephan Roger, Nüesch-Inderbinen Magdalena
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Front Vet Sci. 2019 Feb 5;6:17. doi: 10.3389/fvets.2019.00017. eCollection 2019.
We investigated a collection of strains belonging to the - (ACB) complex obtained from a veterinary clinic with regard to their genetic relatedness, presence of antibiotic resistance genes and antimicrobial susceptibility profiles. Fifty-eight ACB-complex strains from animals treated at a veterinary clinic between 2006 and 2017, and seven strains collected from the hospital environment during 2012 were analyzed. Assignment to sequence types (ST) and international complexes (IC) was done by multilocus sequence typing (MLST) according to the Pasteur scheme. Genes encoding carbapenemases, aminoglycoside-modifying enzymes, macrolide-, quinolone- and co-trimoxazole resistance genes, the IS element, virulence associated genes and plasmid associated toxin-antitoxin markers were identified by microarray. Genes encoding -like carbapenemases were amplified by PCR and sequenced. Susceptibility profiles were determined by disc diffusion or by broth microdilution. Among 50 isolates from animals, two predominant clones were observed linked to CC1 ( = 27/54% of the isolates) and CC25 ( = 14/28%), respectively. Strains of IC I harbored , ', and genes. Isolates belonging to CC25 possessed . Six (12%) isolates belonging to CC2 and carrying were also noted. One isolate belonged to CC10 ( ), one to CC149 ( ), the remaining isolate was assigned to ST1220 and possessed . Of six environmental , four (66.7%) belonged to CC25 ( ), one (16.7%) to CC2 ( ) and one to CC3 ( ). Nine isolates (eight from animals and one environmental strain) were non- strains and did not harbor -like genes. None of the isolates carried , , or , and none were resistant to carbapenems. Clonal lineages of the veterinary isolates in our collection are identical to those globally emerging in humans but do not harbor . CC25 may be specific for this particular veterinary clinic environment.
我们调查了从一家兽医诊所获得的属于 - (ACB) 复合体的一系列菌株，研究了它们的遗传相关性、抗生素抗性基因的存在情况以及抗菌药敏谱。分析了2006年至2017年间在一家兽医诊所接受治疗的动物身上分离出的58株ACB复合体菌株，以及2012年从医院环境中收集的7株菌株。根据巴斯德方案，通过多位点序列分型 (MLST) 确定序列类型 (ST) 和国际复合体 (IC)。通过微阵列鉴定编码碳青霉烯酶、氨基糖苷修饰酶、大环内酯类、喹诺酮类和复方新诺明抗性基因、IS元件、毒力相关基因和质粒相关毒素-抗毒素标记的基因。通过PCR扩增并测序编码 - 样碳青霉烯酶的基因。通过纸片扩散法或肉汤微量稀释法测定药敏谱。在50株动物分离株中，观察到两个主要克隆分别与CC1（= 27/54% 的分离株）和CC25（= 14/28%）相关。IC I的菌株携带 、' 和 基因。属于CC25的分离株拥有 。还注意到6株（12%）属于CC2并携带 的分离株。1株分离株属于CC10（ ），1株属于CC149（ ），其余分离株被指定为ST1220并拥有 。在6株环境菌株中，4株（66.7%）属于CC25（ ），1株（16.7%）属于CC2（ ），1株属于CC3（ ）。9株分离株（8株来自动物，1株环境菌株）是非 菌株，不携带 - 样基因。所有分离株均未携带 、 或 ，且均对碳青霉烯类药物不耐药。我们收集的兽医分离株的克隆谱系与全球人类中出现的克隆谱系相同，但不携带 。CC25可能是这个特定兽医诊所环境所特有的。
