The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Normal University, No. 100, Guilin Road, Xuhui District, Shanghai, 200234, PR China; Musculoskeletal Research Laboratory, Department of Orthopaedics & Traumatology Faculty of Medicine, The Chinese University of Hong Kong, No. 437, Ma Liu Shui, Shatin, New Territories, Hong Kong SAR, 999077, PR China.
Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639, Zhizaoju Road, Huangpu District, Shanghai, 200011, PR China.
Biomaterials. 2022 Sep;288:121751. doi: 10.1016/j.biomaterials.2022.121751. Epub 2022 Aug 21.
Endowing biomaterials with functional elements enhances their biological properties effectively. However, improving bioactivity and biosafety simultaneously is still highly desirable. Herein, cerium (Ce) and copper (Cu) are incorporated into silicocarnotite (CPS) to modulate the constitution and microstructure for degradability, bioactivity and biosafety regulation. Our results demonstrated that introducing Ce suppressed scaffold degradation, while, co-incorporation of both Ce and Cu accelerated degradability. Osteogenic effect of CPS in vitro was promoted by Ce and optimized by Cu, and Ce-induced angiogenic inhibition could be mitigated by cell coculture method and reversed by Ce-Cu co-incorporation. Ce enhanced osteogenic and angiogenic properties of CPS in a dose-dependent manner in vivo, and Cu-Ce coexistence exhibited optimal bioactivity and satisfactory biosafety. This work demonstrated that coculture in vitro was more appropriately reflecting the behavior of implanted biomaterials in vivo. Interactive effects of multi-metal elements were promising to enhance bioactivity and biosafety concurrently. The present work provided a promising biomaterial for bone repair and regeneration, and offered a comprehensive strategy to design new biomaterials which aimed at adjustable degradation behavior, and enhanced bioactivity and biosafety.
赋予生物材料功能性元素可以有效地增强其生物学特性。然而，同时提高生物活性和生物安全性仍然是非常需要的。在此，我们将铈（Ce）和铜（Cu）掺入硅钙石（CPS）中，以调节其组成和微观结构，从而调节降解性、生物活性和生物安全性。研究结果表明，引入 Ce 可以抑制支架降解，而同时掺入 Ce 和 Cu 则可以加速降解。Ce 和 Cu 可以协同促进 CPS 的体外成骨作用，而 Ce 诱导的血管生成抑制可以通过细胞共培养方法来减轻，并通过 Ce-Cu 共掺入来逆转。Ce 以剂量依赖的方式增强了 CPS 在体内的成骨和血管生成特性，而 Cu-Ce 共存则表现出最佳的生物活性和良好的生物安全性。本研究表明，体外共培养更能反映植入生物材料在体内的行为。多金属元素的相互作用有望同时增强生物活性和生物安全性。本研究为骨修复和再生提供了一种有前途的生物材料，并为设计具有可调降解行为、增强生物活性和生物安全性的新型生物材料提供了一种全面的策略。
