外泌体 miRNAs 和膜蛋白在急性 HIV 感染者中的潜在作用。
The potential role of exosomal miRNAs and membrane proteins in acute HIV-infected people.
机构信息
Institute of Infectious Diseases of Integrated Traditional Chinese and Western Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China.
General Surgery Center, Beijing Youan Hospital, Capital Medical University, Beijing, China.
出版信息
Front Immunol. 2022 Aug 12;13:939504. doi: 10.3389/fimmu.2022.939504. eCollection 2022.
Exosomes play an important role during human immunodeficiency virus (HIV) acute infection. Yet, information regarding its cargo and its association with HIV rapid progressors (RPs) and typical progressors (TPs) remain largely unknown. In this study, exosomal miRNAs sequencing and mass cytometry were used to identify differential exosomal miRNAs and membrane proteins that participate in the pathogenesis of TPs and RPs. We discovered that miR-144-5p, miR-1180-3p, miR-451a, miR-362-5p, and miR-625-5p are associated with the TPs and miR-362-5p with the RPs. Decreased autophagy, amino acid metabolism, immune response, and IL-6 are closely related to RPs. In addition, SP1 was selected as the most significant transcription factor (TF) associated with disease progression. CD49D, CD5, CCR5, CD40, CD14, and CD86 were selected as the differential exosomal membrane proteins between TPs and RPs. This study provides valuable information for clarifying the mechanism in people with acute HIV infection.
外泌体在人类免疫缺陷病毒 (HIV) 急性感染中发挥着重要作用。然而，关于其携带物以及与 HIV 快速进展者 (RPs) 和典型进展者 (TPs) 的关系的信息在很大程度上仍不清楚。在这项研究中，我们使用外泌体 miRNA 测序和质谱流式细胞术来鉴定参与 TPs 和 RPs 发病机制的差异外泌体 miRNA 和膜蛋白。我们发现 miR-144-5p、miR-1180-3p、miR-451a、miR-362-5p 和 miR-625-5p 与 TPs 相关，miR-362-5p 与 RPs 相关。减少自噬、氨基酸代谢、免疫反应和 IL-6 与 RPs 密切相关。此外，SP1 被选为与疾病进展最相关的重要转录因子 (TF)。CD49D、CD5、CCR5、CD40、CD14 和 CD86 被选为 TPs 和 RPs 之间差异的外泌体膜蛋白。这项研究为阐明急性 HIV 感染人群的发病机制提供了有价值的信息。
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