and Studies of Soybean Peptides on Milk Production, Rumen Fermentation, Ruminal Bacterial Community, and Blood Parameters in Lactating Dairy Cows.

Soybean peptides (SPs), a feed additive derived from soybean, exhibit nutritional function and biological activity in monogastric animals, but limited studies have been conducted in dairy cows. Our experiments were conducted to evaluate the effects of SPs on the nutrient degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) and milk production, rumen fermentation and bacterial community, and blood parameters of dairy cows. For experiment, ruminal fluids were collected from three ruminal cannulated Holstein dairy cows. A total of three levels of SPs (0, 0.38, and 1.92 g/kg DM of SPs) were added to the total mixed ration (TMR). Nutrient degradability and fermentation fluid pH were determined at 24 and 48 h using 3.0 g samples of the substrate. Gas production after 48 h was recorded by an automated trace gas recording system using 0.5 g samples of the substrate. The results showed that DM, NDF, ADF ( < 0.01), and CP ( < 0.05) degradabilities were significantly increased at 1.92 g/kg DM of SPs at 24 h, and asymptotic gas production ( = 0.05) was increased at 48 h. For experiment, 110 lactating Holstein cows (209.7 ± 65.2 DIM; 37.2 ± 6.4 kg/d milk yield) were randomly assigned to 0 (control group, CON) or 50 g/head/day SPs (SP-supplemented group). Yields of milk ( < 0.05), milk protein ( < 0.05), and milk lactose (0.05 < < 0.10) increased on SPs supplementation; however, the milk fat percentage decreased ( < 0.05). The concentrations of individual volatile fatty acids (VFAs) ( < 0.05) and superoxide dismutase (SOD) ( < 0.01) were also increased. Rumen bacterial diversity in SP-supplemented cows was higher ( < 0.05). The relative abundances of , and were significantly increased and that of was decreased ( < 0.05). Our results showed that supplementing 1.92 g/kg DM of SPs could improve the nutrient degradability and 50 g/head/day of SPs could improve milk production and antioxidant ability of dairy cows. The rumen bacterial diversity was also enhanced by SP supplementation.