Ueda Koichi, Lee Yuumi, Inomata Yosuke, Taniguchi Kohei, Uchiyama Kazuhisa, Ito Yuko
Department of Plastic and Reconstructive Surgery, Osaka Medical and Pharmaceutical University, Osaka, Japan.
Department of General and Gastroenterological Surgery, Osaka Medical and Pharmaceutical University, Osaka, Japan.
Plast Reconstr Surg Glob Open. 2022 Aug 24;10(8):e4492. doi: 10.1097/GOX.0000000000004492. eCollection 2022 Aug.
We previously reported that keloid nodules had such specific structures that higher expression of autophagy proteins and glycolytic markers was observed in the central zone fibroblasts than in marginal zone fibroblasts. The purpose of this study is to investigate how keloid nodules play a role in metabolic activity for continuous expansion.
A total of 57 nodules were randomly chosen from seven keloid samples and divided into four groups of disease duration (2, 4, 6, and 17 years). Immunohistochemical and immunofluorescent analyses were performed.
Immunohistochemical analysis with anti-CD-31 confirmed that the nodules had a structure with a greater number of vessels in the marginal zone than in the central zone. The density of fibroblasts in nodules (190.29 ± 64.45) was significantly higher than that of surrounding connective tissue (140.18 ± 63.94) ( < 0.05).The area of each nodule increased for 2 to 4 years, then decreased, graphically represented by an approximately horizontal line, to 17 years. The ratio of total nodule area/dermis area increased as disease duration lengthened. The maximum ratio was the 17-year group at 48.01%. The nodule number/dermis area ratio rose approximately with advancing disease duration.
Instead of increasing the size of the nodules, their number and total area increased within the keloid lesions. We believe that the keloid nodules must play an important role in energy metabolic activity for continuous growth by increasing in number and total area.
我们之前报道过，瘢痕疙瘩结节具有特定结构，中央区成纤维细胞中自噬蛋白和糖酵解标志物的表达高于边缘区成纤维细胞。本研究的目的是探讨瘢痕疙瘩结节如何在代谢活动中发挥作用以实现持续扩张。
从7个瘢痕疙瘩样本中随机选取57个结节，分为疾病病程的四组（2年、4年、6年和17年）。进行免疫组织化学和免疫荧光分析。
抗CD-31免疫组织化学分析证实，结节结构中边缘区的血管数量多于中央区。结节中成纤维细胞密度（190.29±64.45）显著高于周围结缔组织（140.18±63.94）（<0.05）。每个结节的面积在2至4年增加，然后下降，以近似水平线图形表示，直至17年。结节总面积/真皮面积的比例随着疾病病程延长而增加。最大比例是17年组，为48.01%。结节数量/真皮面积比例大致随着疾病病程进展而上升。
瘢痕疙瘩病变内结节的数量和总面积增加，而非结节大小增加。我们认为，瘢痕疙瘩结节必定通过增加数量和总面积在能量代谢活动中对持续生长发挥重要作用。
