Makerere University Hospital, Kampala, Uganda.
Department of Medical Microbiology, School of Biomedical Sciences, Makerere. University College of Health Sciences, Kampala, Uganda.
Afr Health Sci. 2022 Mar;22(1):581-588. doi: 10.4314/ahs.v22i1.67.
Diarrhoeagenic (DEC) is a leading cause of childhood diarrhoea. This study estimated the prevalence of DEC and DEC pathotypes among children with acute diarrhoea in Southern Uganda.
A cross-sectional study was conducted on 267 children less than 5 years with acute diarrhoea, admitted to Rakai General Hospital in Southern Uganda. Faecal samples were collected from the children and processed for isolation of . The presence of DEC and the distribution of DEC pathotypes were determined by polymerase chain reaction.
A total of 102 (38.2%, 102/267) children had DEC of various pathotypes - enteroaggregative (EAEC) (14.2%); enteropathogenic E. coli (EPEC) (6.7%); enterotoxigenic (ETEC) (6%); enteroinvasive (EIEC) (7.5%); enterohemorrhagic (EHEC) (3%); and cell-detaching E. coli (CDEC) (0.75%). The difference in the overall prevalence of DEC was not significant regarding HIV but individually, EAEC and CDEC were associated with HIV-positive status while ETEC was associated with HIV-negative status.
DEC is prevalent in children with acute diarrhoea in Southern Uganda and its identification in children should be considered among strategies for combatting childhood diarrhoea in Africa.
肠致病性（DEC）是导致儿童腹泻的主要原因。本研究旨在评估乌干达南部地区急性腹泻儿童中 DEC 和 DEC 病原体的流行情况。
在乌干达南部的 Rakai 综合医院，对 267 名年龄小于 5 岁的急性腹泻儿童进行了一项横断面研究。收集儿童的粪便样本并进行处理，以分离 。通过聚合酶链反应确定 DEC 和 DEC 病原体的存在和分布。
共有 102 名（38.2%，102/267）儿童患有各种 DEC 病原体，包括肠聚集性大肠杆菌（EAEC）（14.2%）、肠致病性大肠杆菌（EPEC）（6.7%）、肠毒性大肠杆菌（ETEC）（6%）、肠侵袭性大肠杆菌（EIEC）（7.5%）、肠出血性大肠杆菌（EHEC）（3%）和细胞脱落性大肠杆菌（CDEC）（0.75%）。在 HIV 方面，DEC 的总体流行率没有显著差异，但 EAEC 和 CDEC 与 HIV 阳性状态相关，而 ETEC 与 HIV 阴性状态相关。
DEC 在乌干达南部急性腹泻儿童中很常见，在非洲防治儿童腹泻的策略中应考虑对 DEC 的识别。
