Microbiology unit, Department of medical laboratory science faculty of allied health science, Bayero University kano, Nigeria, P.M.B 3011, Kano, Nigeria.
Federal Medical Centre Katsina, PMB 2121, Katsina, Nigeria.
Afr Health Sci. 2022 Mar;22(1):98-105. doi: 10.4314/ahs.v22i1.13.
HPV 16 is the primary etiologic agent of cervical cancer and the presence of L1 and E6 oncoproteins are largely responsible for its virulence. It was the objective of this study to identify HPV16 isolates from suspected cases of cervical cancer at Specialist Hospital Sokoto and Sir Yahaya Memorail Hospiatal Birnin Kebbi, Nigeria and also to identify potent HPV16's L1 protein inhibitor using in silico analysis.
A total of 144 cervical samples consisting of 21 low grade squamous intraepithelial lesion, 6 high grade lesion and 117 negative pap smears were collected. The samples were subjected for molecular detection using PCR targeting E6 gene of the virus. Data generated for the molecular prevalence was statistically analyzed using Chi-square method. AutoDock Vina was used to carry out the molecular docking between 2hr5 and Chicoric acid, curcumin and Echinacoside.
Out of the 144 samples, 24 samples were positive for the PCR representing 16.9% molecular prevalence rate. There is statistically significant association between cyto-diagnoses and presence of HPV16 (P < 0.05). Docking analysis showed that the Chicoric acid components of Echinacea purpurae have strong binding affinity (-8.7 kcal/mol) to the L1 protein of the HPV.
This study provides data on HPV 16 epidemiology in northern Nigeria, and also provides novel evidence for consideration on certain interacting residues, when synthesizing Anti-HPV compounds in the wet lab.
HPV16 是宫颈癌的主要病因，其 L1 和 E6 癌蛋白的存在在很大程度上与其毒力有关。本研究的目的是鉴定尼日利亚索科托专家医院和锡亚亚·雅哈亚纪念医院疑似宫颈癌病例中的 HPV16 分离株，并利用计算机分析鉴定有效的 HPV16 L1 蛋白抑制剂。
共采集 144 例宫颈样本，包括 21 例低级别鳞状上皮内病变、6 例高级别病变和 117 例阴性巴氏涂片。采用针对病毒 E6 基因的 PCR 法对样本进行分子检测。采用卡方检验对分子流行率数据进行统计学分析。利用 AutoDock Vina 对 Chicoric acid、curcumin 和 Echinacoside 与 2hr5 之间进行分子对接。
144 个样本中，24 个样本的 PCR 呈阳性，代表 16.9%的分子流行率。细胞诊断与 HPV16 之间存在统计学显著关联（P < 0.05）。对接分析表明，紫锥菊 Chicoric acid 成分与 HPV 的 L1 蛋白具有很强的结合亲和力（-8.7 kcal/mol）。
本研究提供了尼日利亚北部 HPV16 流行病学的数据，并且在湿实验室合成抗 HPV 化合物时，为某些相互作用的残基提供了新的考虑证据。
