Department of Pathophysiology, Translational Medicine Institute, Shenyang Medical College, Shenyang, China.
Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Scand J Immunol. 2021 Mar;93(3):e13003. doi: 10.1111/sji.13003. Epub 2020 Dec 11.
It was reported that the expression of Toll-like receptor (TLR) 9 may be related to Th2-type allergic inflammation including allergic rhinitis (AR). However, little is known about the expression of TLR9 in the basophils in AR. In the present study, the expression of TLR9 was examined by flow cytometry analysis, and the expression of TLR9 mRNA in KU812 was determined by quantitative real-time PCR. The results showed that the percentage of TLR9 CCR3 cells in blood granulocytes increased by 46% in patients with AR, but not in peripheral blood mononuclear cells (PBMCs). Allergens namely Dermatophagoide allergen extract (DAE) and Platanus pollen allergen extract (PPAE) upregulated the expression of TLR9 in CCR3 granulocytes by 76% and 84%, respectively. DAE and PPAE also enhanced the proportions of TLR9 CD123 HLA-DR cells and TLR9 CCR3 CD123 HLA-DR cells in granulocytes and PBMCs of patients with AR. In order to investigate the actions of allergens on basophils, KU812 cells were used. It was observed that all KU812 cells expressed TLR9, and the expression intensity of TLR9 in a single KU812 cell was elevated by CpG. IL-37, IL-31, IL-33, Artemisia sieversiana wild allergen extract (ASWAE), DAE, OVA and Der p 1 induced an increase in the expression of TLR9 mRNA and IL-6 production in KU812 cells. It was shown that the percentage of TLR9-expressing basophils increased in the blood of ovalbumin (OVA)-sensitized mice. In conclusion, an increased expression of TLR9 and the production of IL-6 in basophils implicate that the contribution of basophils to AR is likely via TLR9.
据报道，Toll 样受体（TLR）9 的表达可能与包括过敏性鼻炎（AR）在内的 Th2 型过敏炎症有关。然而，TLR9 在 AR 中的嗜碱性粒细胞中的表达知之甚少。在本研究中，通过流式细胞术分析检测 TLR9 的表达，并通过定量实时 PCR 确定 KU812 中 TLR9 mRNA 的表达。结果表明，AR 患者血液粒细胞中 TLR9 CCR3 细胞的百分比增加了 46%，但外周血单核细胞（PBMCs）中没有增加。过敏原如嗜酸性真菌过敏原提取物（DAE）和悬铃木花粉过敏原提取物（PPAE）分别使 CCR3 粒细胞中 TLR9 的表达上调了 76%和 84%。DAE 和 PPAE 还增强了 AR 患者粒细胞和 PBMC 中 TLR9 CD123 HLA-DR 细胞和 TLR9 CCR3 CD123 HLA-DR 细胞的比例。为了研究过敏原对嗜碱性粒细胞的作用，使用了 KU812 细胞。观察到所有 KU812 细胞均表达 TLR9，并且 CpG 可提高单个 KU812 细胞中 TLR9 的表达强度。IL-37、IL-31、IL-33、蒿属植物野生过敏原提取物（ASWAE）、DAE、OVA 和 Der p 1 诱导 KU812 细胞中 TLR9 mRNA 的表达增加和 IL-6 的产生。结果表明，OVA 致敏小鼠血液中表达 TLR9 的嗜碱性粒细胞百分比增加。总之，嗜碱性粒细胞中 TLR9 的表达增加和 IL-6 的产生表明嗜碱性粒细胞可能通过 TLR9 参与 AR。
