变应性鼻炎患者中 CD123+HLA-DR 粒细胞增多及变应原对细胞膜标志物表达的影响。
Increased CD123 HLA-DR Granulocytes in Allergic Rhinitis and Influence of Allergens on Expression of Cell Membrane Markers.
机构信息
The Respiratory and Allergic Disease Diagnosing Management Center of the PLA. General Hospital of Northern Theater Command, Shenyang, Liaoning, China.
Translational Medicine Research Centre, Shenyang Medical College, Shenyang, Liaoning, China.
出版信息
Am J Rhinol Allergy. 2024 Sep;38(5):294-305. doi: 10.1177/19458924241252456. Epub 2024 May 7.
BACKGROUND
It is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes.
OBJECTIVE
To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.
METHODS
Flow cytometry was used to detect the expression of the membrane molecules.
RESULTS
While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.
CONCLUSIONS
The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.
背景
据报道，外周血单个核细胞（PBMC）中 CD123+HLA-DR-细胞为嗜碱性粒细胞，CD203c、CD63 和 FcεRI 分子是嗜碱性粒细胞的激活标志物。然而，血液粒细胞中 CD123+HLA-DR-细胞的情况知之甚少。
目的
探讨变应性鼻炎（AR）患者血液粒细胞和外周 PBMC 中 CD123+HLA-DR-细胞的存在情况，以及变应原对嗜碱性粒细胞细胞膜标志物的影响。
方法
采用流式细胞术检测细胞膜分子的表达。
结果
虽然 CD123+HLA-DR-PBMC 是嗜碱性粒细胞的代表，但 AR 患者中其数量没有明显变化。相反，季节性 AR（sAR）和常年性 AR（pAR）患者中 CD123+HLA-DR-粒细胞的比例和数量均明显增加，而 CD123+HLA-DR-粒细胞仅占嗜碱性粒细胞的 50%左右。CD123+HLA-DR-粒细胞中的 CD63+、CD203c+和 FcεRIα+细胞也表现出更高的活性。屋尘螨过敏原提取物（HDME）和蒿属植物野生提取物过敏原进一步增加了 sAR 和 pAR 患者粒细胞中 CD123+HLA-DR-细胞以及 pAR 患者 PBMC 中 CD123+HLA-DR-细胞的数量。
结论
使用 CD123+HLA-DR-粒细胞和 PBMC 可能不足以诊断 AR。变应原可能通过影响 CD123+HLA-DR-细胞的数量以及这些细胞中 CD63、CD203c 和 FcεRIα的表达，从而促进 AR 的发生。
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