伊朗西北部Meshkinshahr县犬类隔离跳蚤中[具体物质]的分子检测
Molecular Detection of in Isolated Fleas of Dogs in Meshkinshahr County, Northwest of Iran.
作者信息
Azarm Amrollah, Dalimi Abdolhossein, Pirestani Majid, Mohammadiha Anita
机构信息
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran J Parasitol. 2022 Apr-Jun;17(2):159-166. doi: 10.18502/ijpa.v17i2.9531.
BACKGROUND
Fleas (Insecta: Siphonaptera) are considered as highly specialized bloodsucking on mammals such as humans, livestock, dog, cat, rabbit, squirrels, rats, and mice. The desire for blood feeding from warm-blooded animals has led to becoming an intermediate host for some tapeworms like . The aim of this study was to detect larval infection in fleas of dogs living in Mesh-kinshahr County, northwest of Iran.
METHODS
Fleas were collected from 42 dogs using brushing the hair in Meshkinshahr for one year (2014-2015). After the morphological study, fleas were preserved in 90% ethanol for molecular identification. After DNA extraction, the 28S ribosomal RNA gene (∼670 bp) of was amplified using specific primers. Finally, the PCR products were sequenced.
RESULTS
Overs, 974 fleas were collected from the dogs. In the morphological study, three species , and were identified. PCR and sequence analysis results showed that 4 isolates were infected with . Also, no positive specimens were isolated from the other two species.
CONCLUSION
is reported as the most important species of fleas in transferring in that region.
背景
跳蚤（昆虫纲：蚤目）被认为是高度特化的以人类、家畜、狗、猫、兔子、松鼠、大鼠和小鼠等哺乳动物为宿主的吸血昆虫。对温血动物血液的吸食欲望导致其成为某些绦虫的中间宿主，如 。本研究的目的是检测生活在伊朗西北部Mesh-kinshahr县的狗身上跳蚤的 幼虫感染情况。
方法
在Meshkinshahr通过梳理毛发从42只狗身上收集跳蚤，为期一年（2014 - 2015年）。经过形态学研究后，将跳蚤保存在90%乙醇中用于分子鉴定。提取DNA后，使用特异性引物扩增 的28S核糖体RNA基因（约670 bp）。最后，对PCR产物进行测序。
结果
从狗身上共收集到974只跳蚤。在形态学研究中，鉴定出三种跳蚤，即 、 和 。PCR和序列分析结果显示，4个分离株感染了 。此外，从其他两个物种中未分离到阳性标本。
结论
据报道， 在该地区是传播 最重要的跳蚤种类。
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