Stafford Lauren, Valcarce Vivian, Henry Matthew, Neu Josef, Parker Leslie, Martina Mueller, Vicuna Valeria, Gowen Taylor, Cato Emilee, Kosik Ivan, Yewdell Jonathan, Atkinson Mark, Cacho Nicole, Li Nan, Larkin Joseph
University of Florida.
National Institutes of Health/National Institute of Allergy and Infectious Diseases.
Res Sq. 2022 Aug 19:rs.3.rs-1950944. doi: 10.21203/rs.3.rs-1950944/v1.
Assess the presence, durability, and neutralization capacity of SARS-CoV-2-specific antibodies in breastfeeding infants' stools, mother's plasma, and human milk following maternal vaccination. Thirty-seven mothers and 25 infants were enrolled between December 2020 and November 2021 for this prospective observational study. Human milk, maternal plasma, and infants' stools were collected pre-vaccination and at periods up to 6 months following COVID-19 vaccine series initiation/completion. SARS-CoV-2 antibody levels and their neutralization capacities were assessed in collected samples. SARS-CoV-2-specific IgA and IgG levels were higher in infant stool post-maternal vaccination amongst milk-fed compared to pre-COVID controls. Human milk and plasma SARS-CoV-2-specific IgA and IgG concentrations decreased over 6 months post-vaccination but remained higher than pre-vaccination levels. We observed improved neutralization capacity in milk antibodies over time. The presence of neutralizing SARS-CoV-2-specific antibodies in infant stool following maternal vaccination offers further evidence of the lasting transfer of these antibodies through breastfeeding and their protective effect.
评估产妇接种疫苗后，母乳喂养婴儿粪便、母亲血浆和母乳中新冠病毒特异性抗体的存在情况、持久性及中和能力。2020年12月至2021年11月期间，招募了37名母亲和25名婴儿参与这项前瞻性观察研究。在接种疫苗前以及启动/完成新冠疫苗系列接种后的6个月内，收集母乳、母亲血浆和婴儿粪便。对收集的样本评估新冠病毒抗体水平及其中和能力。与新冠疫情前的对照组相比，母乳喂养婴儿在母亲接种疫苗后粪便中的新冠病毒特异性IgA和IgG水平更高。接种疫苗后6个月内，母乳和血浆中新冠病毒特异性IgA和IgG浓度下降，但仍高于接种疫苗前的水平。随着时间推移，我们观察到母乳抗体的中和能力有所提高。产妇接种疫苗后婴儿粪便中存在中和新冠病毒特异性抗体，进一步证明了这些抗体通过母乳喂养持续传递及其保护作用。
