《L. var. argentea Andre 叶的水提物和有机溶剂提取物的民族药理学调查、矿物和化学成分、抗氧化和抗菌活性》。
Ethnopharmacological Survey, Mineral and Chemical Content, Antioxidant, and Antibacterial Activities of Aqueous and Organic Extracts of L. var. argentea Andre Leaves.
机构信息
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life (SNAMOPEQ), Polydisciplinary Faculty of Taza (FPT), Sidi Mohamed Ben Abdellah University (USMBA) of Fez, B.P.: 1223 Taza-Gare, Taza, Morocco.
出版信息
Biomed Res Int. 2022 Aug 5;2022:1091247. doi: 10.1155/2022/1091247. eCollection 2022.
INTRODUCTION
The present study is carried out for the first time on L. var. argentea Andre from the region of Taza using an ethnopharmacological survey, an experimental study of the mineralogical and chemical compositions, and evaluations of the antioxidant and antibacterial activities.
METHODS
After conducting the ethnopharmacological survey, a mineralogical and phytochemical study involving the preparation of aqueous and organic extracts was done. Essential oils were also extracted by hydrodistillation. Subsequently, qualitative and quantitative chemical analyses were performed. evaluation of antioxidant activities was performed by five tests (HO, DPPH, ABTS, FRAP, and RP) and antibacterial activities by the disc method and determination of MIC and MBC. A principal component analysis (PCA) was performed to visualize the different correlations.
RESULTS
The different parts of the plant are used for the treatment of digestive disorders, cardiovascular diseases, and diabetes. In addition, the leaves are rich in mineral compounds, catechic tannins, flavonoids, and sterols. However, they have some traces of essential oils. The quantitative analysis revealed that the ethanolic macerated had a higher content of total polyphenols (100.27 ± 1.95 mg EAG/g E) and catechic tannins (52.11 ± 1.02 mg EC/g E). This extract had a strong antioxidant capacity (HO (37.34 ± 0.55%), DPPH (IC = 31.18 ± 0.66 g/ml), ABTS (108.28 ± 1.29 mg E AA/g E), FRAP (148.85 ± 0.43 mg E T/g E), and RP (10.86 ± 0.01 mg E AA/g E). The same extract had a bactericidal effect against . Principal component analysis (PCA) showed that antioxidant activity was highly correlated with the chemical composition of leaves; a high correlation was recorded between the total polyphenol content and ABTS ( = 0.9779), FRAP ( = 0.9644), DPPH ( = 0.9418), and PR ( = 0.9271) tests. In addition, cathectic tannins were highly correlated with the tests of DPPH ( = 0.9753) and ABTS ( = 0.8843). Flavonoids were similarly correlated with DPPH ( = 0.8897) and ABTS ( = 0.7599) tests.
CONCLUSION
These results could justify the traditional use of the leaves of in the region of Taza for the treatment of some diseases.
简介
本研究首次对来自塔扎地区的 L. var. argentea Andre 进行了研究，采用了民族药理学调查、矿物学和化学成分的实验研究以及抗氧化和抗菌活性评估。
方法
在进行民族药理学调查后，进行了矿物学和植物化学研究，包括制备水提物和有机提取物。还通过水蒸馏提取了精油。随后进行了定性和定量化学分析。通过五种测试（HO、DPPH、ABTS、FRAP 和 RP）评估抗氧化活性，通过圆盘法和 MIC 和 MBC 测定评估抗菌活性。进行了主成分分析（PCA）以可视化不同的相关性。
结果
植物的不同部位用于治疗消化疾病、心血管疾病和糖尿病。此外，叶子富含矿物化合物、儿茶单宁、类黄酮和甾醇。然而，它们含有一些精油的痕迹。定量分析显示，醇提物中总多酚（100.27 ± 1.95mg EAG/g E）和儿茶单宁（52.11 ± 1.02mg EC/g E）含量较高。该提取物具有很强的抗氧化能力（HO（37.34 ± 0.55%）、DPPH（IC=31.18 ± 0.66g/ml）、ABTS（108.28 ± 1.29mg E AA/g E）、FRAP（148.85 ± 0.43mg E T/g E）和 RP（10.86 ± 0.01mg E AA/g E）。相同的提取物对 具有杀菌作用。主成分分析（PCA）表明，抗氧化活性与 叶子的化学成分高度相关；总多酚含量与 ABTS（=0.9779）、FRAP（=0.9644）、DPPH（=0.9418）和 PR（=0.9271）测试高度相关。此外，儿茶单宁与 DPPH（=0.9753）和 ABTS（=0.8843）测试高度相关。类黄酮与 DPPH（=0.8897）和 ABTS（=0.7599）测试也有类似的相关性。
结论
这些结果可以证明塔扎地区 叶子用于治疗某些疾病的传统用途是合理的。
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