Hobin Michael, Dorfman Katherine, Adel Mohamed, Rivera-Rodriguez Emmanuel J, Kuklin Elena A, Ma Dingbang, Griffith Leslie C
Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA.
Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454-9110, USA.
iScience. 2022 Aug 1;25(9):104874. doi: 10.1016/j.isci.2022.104874. eCollection 2022 Sep 16.
Sleep circuitry evolved to have both dedicated and context-dependent modulatory elements. Identifying modulatory subcircuits and understanding their molecular machinery is a major challenge for the sleep field. Previously, we identified 25 sleep-regulating microRNAs in , including the developmentally important microRNA . Here we show that acts in the adult to promote early nighttime sleep through a population of glutamatergic neurons that is intimately involved in applying contextual information to behaviors, the γ5β'2a/β'2mp/β'2mp_bilateral Mushroom Body Output Neurons (MBONs). Calcium imaging revealed that bantam inhibits the activity of these cells during the early night, but not the day. Blocking synaptic transmission in these MBONs rescued the effect of knockdown. This suggests promotes early night sleep via inhibition of the γ5β'2a/β'2mp/β'2mp_bilateral MBONs. RNAseq identifies and as possible mediators, establishing a new role for as an active regulator of sleep and neural activity in the adult fly.
睡眠神经回路进化出了既有专门的又有依赖于环境的调节元件。识别调节性子回路并了解其分子机制是睡眠领域的一项重大挑战。此前，我们在[具体内容未给出]中鉴定出了25种调节睡眠的微小RNA，包括在发育中具有重要作用的微小RNA[具体名称未给出]。在此，我们表明[具体名称未给出]在成体中通过一群谷氨酸能神经元发挥作用，促进夜间早期睡眠，这群神经元密切参与将环境信息应用于行为，即γ5β'2a/β'2mp/β'2mp双侧蘑菇体输出神经元（MBONs）。钙成像显示，bantam在夜间早期抑制这些细胞的活性，但在白天不抑制。阻断这些MBONs中的突触传递挽救了[具体名称未给出]敲低的效果。这表明[具体名称未给出]通过抑制γ5β'2a/β'2mp/β'2mp双侧MBONs来促进夜间早期睡眠。RNA测序将[具体名称未给出]和[具体名称未给出]鉴定为可能的介导因子，确立了[具体名称未给出]在成年果蝇中作为睡眠和神经活动的主动调节因子的新作用。
