Laboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Manaus 69027-070, Brazil.
Programa de Pós-Graduação em Biologia Parasitária, Instituto Oswaldo Cruz (IOC), Rio de Janeiro 21040-900, Brazil.
Genes (Basel). 2024 Aug 8;15(8):1046. doi: 10.3390/genes15081046.
and are responsible for transmitting major human arboviruses such as Dengue, Zika, and Chikungunya, posing a global threat to public health. The lack of etiological treatments and efficient vaccines makes vector control strategies essential for reducing vector population density and interrupting the pathogen transmission cycle. This study evaluated the impact of long-term pyriproxyfen exposure on the genetic structure and diversity of and mosquito populations. The study was conducted in Manaus, Amazonas, Brazil, where pyriproxyfen dissemination stations have been monitored since 2014 up to the present day. Double digest restriction-site associated DNA sequencing was performed, revealing that despite significant local population reductions by dissemination stations with pyriproxyfen in various locations in Brazil, focal intervention has no significant impact on the population stratification of these vectors in urban scenarios. The genetic structuring level of suggests it is more stratified and directly affected by pyriproxyfen intervention, while for exhibits a more homogeneous and less structured population. The results suggest that although slight differences are observed among mosquito subpopulations, intervention focused on neighborhoods in a capital city is not efficient in terms of genetic structuring, indicating that larger-scale pyriproxyfen interventions should be considered for more effective urban mosquito control.
和 是主要的人类虫媒病毒（如登革热、寨卡和基孔肯雅热）的传播媒介，对全球公共卫生构成威胁。由于缺乏病因治疗和有效的疫苗，因此媒介控制策略对于降低媒介种群密度和中断病原体传播周期至关重要。本研究评估了长期吡丙醚暴露对 和 蚊种群遗传结构和多样性的影响。该研究在巴西亚马逊州的玛瑙斯进行，自 2014 年以来一直在监测吡丙醚传播站。进行了双酶切限制位点相关 DNA 测序，结果表明，尽管在巴西各地的传播站使用吡丙醚显著降低了当地的种群数量，但焦点干预对这些城市环境中媒介的种群分层没有显著影响。 的遗传结构水平表明它受到吡丙醚干预的影响更大，而 则表现出更均匀和较少分层的种群。结果表明，尽管观察到蚊子亚种群之间存在细微差异，但针对首府社区的干预在遗传结构方面效率不高，这表明应考虑更大规模的吡丙醚干预，以实现更有效的城市蚊子控制。
