Long Non-Coding RNA MAFG-AS1 as a Potential Biomarker for Hepatocellular Carcinoma: Linkage with Tumor Features, Markers, Liver Functions, and Survival Profile.

PURPOSE

Long non-coding RNAs musculoaponeurotic fibrosarcoma oncogene family, protein G antisense 1 (lnc-MAFG-AS1) regulates hepatocellular carcinoma (HCC) progression and treatment resistance in multiple ways, while its engagement in HCC clinical management remains obscure. The current study aims to explore the relationship of lnc-MAFG-AS1 with tumor features, liver function indexes, tumor markers, and prognosis in HCC patients.

METHODS

One hundred and fifty-two surgical HCC patients who underwent tumor resection were retrospectively analyzed. Their tumor and adjacent tissues were acquired and then proposed to reverse transcription-quantitative polymerase chain reaction to detect lnc-MAFG-AS1 expression.

RESULTS

Lnc-MAFG-AS1 expression was increased in HCC tumor tissue than in adjacent tissue [median (interquartile range): 2.730 (1.685-4.198) vs. 0.990 (0.703-1.468), < 0.001], with a high area under the curve [0.889, 95% confidence interval (CI): 0.854-0.924] to distinguish them via receiver operating characteristic curve analysis. Tumor lnc-MAFG-AS1 was linked with multifocal nodules (< 0.001), increased Barcelona Clinic Liver Cancer (BCLC) stage (= 0.018), and elevated China Liver Cancer (CNLC) stage (= 0.008), which also correlated with an abnormal alpha-fetoprotein (AFP) level (= 0.004), However, lnc-MAFG-AS1 was not linked with other disease conditions, tumor properties, liver function indexes, or tumor markers (all s> 0.05). In addition, patients with a high expression of lnc-MAFG-AS1 exhibited worse overall survival than those with a low expression of lnc-MAFG-AS1 [median (95% CI): 34.0 (24.5-43.5) vs. 48.0 (41.5-54.5) months] (= 0.011), which was further validated by univariate Cox's analysis [hazard ratio (HR) = 1.827, = 0.013] and multivariate Cox's analysis (HR = 1.697, = 0.040).

CONCLUSION

Lnc-MAFG-AS1 relates to multifocal nodules, increased BCLC stage, elevated CNLC stage, and abnormal AFP level and predicts pejorative prognosis in HCC patients.