Tian Yuanyuan, Wang Jiao, Tian Ge, Li Bing, Chen Moli, Sun Xiaoning
Department of Gastroenterology, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.
Department of Infectious Diseases, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.
Front Surg. 2022 May 17;9:848831. doi: 10.3389/fsurg.2022.848831. eCollection 2022.
Long non-coding RNAs musculoaponeurotic fibrosarcoma oncogene family, protein G antisense 1 (lnc-MAFG-AS1) regulates hepatocellular carcinoma (HCC) progression and treatment resistance in multiple ways, while its engagement in HCC clinical management remains obscure. The current study aims to explore the relationship of lnc-MAFG-AS1 with tumor features, liver function indexes, tumor markers, and prognosis in HCC patients.
One hundred and fifty-two surgical HCC patients who underwent tumor resection were retrospectively analyzed. Their tumor and adjacent tissues were acquired and then proposed to reverse transcription-quantitative polymerase chain reaction to detect lnc-MAFG-AS1 expression.
Lnc-MAFG-AS1 expression was increased in HCC tumor tissue than in adjacent tissue [median (interquartile range): 2.730 (1.685-4.198) vs. 0.990 (0.703-1.468), < 0.001], with a high area under the curve [0.889, 95% confidence interval (CI): 0.854-0.924] to distinguish them via receiver operating characteristic curve analysis. Tumor lnc-MAFG-AS1 was linked with multifocal nodules (< 0.001), increased Barcelona Clinic Liver Cancer (BCLC) stage (= 0.018), and elevated China Liver Cancer (CNLC) stage (= 0.008), which also correlated with an abnormal alpha-fetoprotein (AFP) level (= 0.004), However, lnc-MAFG-AS1 was not linked with other disease conditions, tumor properties, liver function indexes, or tumor markers (all s> 0.05). In addition, patients with a high expression of lnc-MAFG-AS1 exhibited worse overall survival than those with a low expression of lnc-MAFG-AS1 [median (95% CI): 34.0 (24.5-43.5) vs. 48.0 (41.5-54.5) months] (= 0.011), which was further validated by univariate Cox's analysis [hazard ratio (HR) = 1.827, = 0.013] and multivariate Cox's analysis (HR = 1.697, = 0.040).
Lnc-MAFG-AS1 relates to multifocal nodules, increased BCLC stage, elevated CNLC stage, and abnormal AFP level and predicts pejorative prognosis in HCC patients.
长链非编码RNA肌肉腱膜纤维肉瘤癌基因家族蛋白G反义1（lnc-MAFG-AS1）通过多种方式调节肝细胞癌（HCC）的进展和治疗耐药性，但其在HCC临床管理中的作用仍不清楚。本研究旨在探讨lnc-MAFG-AS1与HCC患者肿瘤特征、肝功能指标、肿瘤标志物及预后的关系。
回顾性分析152例行肿瘤切除术的手术HCC患者。获取他们的肿瘤组织和癌旁组织，然后进行逆转录定量聚合酶链反应以检测lnc-MAFG-AS1的表达。
lnc-MAFG-AS1在HCC肿瘤组织中的表达高于癌旁组织[中位数（四分位间距）：2.730（1.685-4.198）对0.990（0.703-1.468），<0.001]，通过受试者工作特征曲线分析，其曲线下面积较高[0.889，95%置信区间（CI）：0.854-0.924]，可用于区分两者。肿瘤lnc-MAFG-AS1与多灶性结节相关（<0.001）、巴塞罗那临床肝癌（BCLC）分期增加（=0.018）和中国肝癌（CNLC）分期升高（=0.008），这也与甲胎蛋白（AFP）水平异常相关（=0.004）。然而，lnc-MAFG-AS1与其他疾病情况、肿瘤特性、肝功能指标或肿瘤标志物均无关联（所有P>0.05）。此外，lnc-MAFG-AS1高表达的患者总生存期比lnc-MAFG-AS1低表达的患者更差[中位数（95%CI）：34.0（24.5-43.5）对48.0（41.5-54.5）个月]（=0.011），单因素Cox分析[风险比（HR）=1.827，=0.013]和多因素Cox分析（HR=1.697，=0.040）进一步验证了这一结果。
lnc-MAFG-AS1与多灶性结节、BCLC分期增加、CNLC分期升高及AFP水平异常相关，并可预测HCC患者的不良预后。
