Izaki Ryosuke, Kobayashi Ayaka, Fujita Hideaki, Harada Kazuhiro, Ozaki Hiroaki, Kadonosono Kazuaki, Uchio Eiichi
Department of Ophthalmology, Fukuoka University School of Medicine, Fukuoka, Japan.
Department of Ophthalmology, Yokohama City University Medical Center, Yokohama, Japan.
J Asthma Allergy. 2022 Aug 22;15:1115-1124. doi: 10.2147/JAA.S375080. eCollection 2022.
We have previously studied clinical and allergological aspects of sick building syndrome (SBS) cases with ocular disorders and found that SBS is suggested to be partially induced by an allergic response. We analyzed the cytokine production profiles of conjunctival and peripheral blood lymphocytes in patients with SBS with ocular manifestations to further evaluate the pathophysiology of SBS from an immunological standpoint.
We obtained conjunctival samples and peripheral blood mononuclear cells (PBMC) from 15 cases of SBS with ocular findings, 49 cases of allergic conjunctival diseases (ACD) (allergic conjunctivitis (AC), atopic keratoconjunctivitis (AKC), and vernal keratoconjunctivitis (VKC)), and normal controls. Frequencies of cytokine-producing T cells were analyzed by flow cytometry based on an intracellular cytokine staining method.
Although no significant difference was observed in the percentage of interferon (IFN)-γ-producing CD4+ T cells in PBMC between patients with SBS and controls, the percentage of interleukin (IL)-4-producing PBMC CD4+ T cells in patients with SBS was significantly higher than that in controls. The percentage of IL-4-producing CD4+ T cells in the conjunctiva in patients with SBS was significantly higher than that in controls, whereas it was significantly lower than that in AKC and VKC. A significant correlation was observed between the percentage of IL-4-producing CD4+ T cells in the conjunctiva and clinical score.
These results suggest that SBS may be a kind of allergic disorder and that IL-4 plays a role in the development of allergic disorders in SBS ocular lesions.
我们之前研究了患有眼部疾病的病态建筑综合征（SBS）病例的临床和过敏学方面，发现SBS可能部分由过敏反应诱发。我们分析了有眼部表现的SBS患者结膜和外周血淋巴细胞的细胞因子产生谱，以从免疫学角度进一步评估SBS的病理生理学。
我们从15例有眼部症状的SBS患者、49例过敏性结膜疾病（ACD）（过敏性结膜炎（AC）、特应性角结膜炎（AKC）和春季角结膜炎（VKC））患者以及正常对照中获取结膜样本和外周血单个核细胞（PBMC）。基于细胞内细胞因子染色方法，通过流式细胞术分析产生细胞因子的T细胞频率。
虽然SBS患者和对照的PBMC中产生干扰素（IFN）-γ的CD4 + T细胞百分比没有显著差异，但SBS患者中产生白细胞介素（IL）-4的PBMC CD4 + T细胞百分比显著高于对照。SBS患者结膜中产生IL-4的CD4 + T细胞百分比显著高于对照，而显著低于AKC和VKC。结膜中产生IL-4的CD4 + T细胞百分比与临床评分之间存在显著相关性。
这些结果表明SBS可能是一种过敏性疾病，并且IL-4在SBS眼部病变的过敏性疾病发展中起作用。
