Cogliati Massimo, Zani Alberto, Rickerts Volker, McCormick Ilka, Desnos-Ollivier Marie, Velegraki Aristea, Escandon Patricia, Ichikawa Tomoe, Ikeda Reiko, Bienvenu Anne-Lise, Tintelnot Kathrin, Tore Okan, Akcaglar Sevim, Lockhart Shawn, Tortorano Anna Maria, Varma Ashok
Lab. Micologia Medica, Dip. Scienze Biomediche per la Salute, Università degli Studi di Milano, Milano, Italy.
Lab. Micologia Medica, Dip. Scienze Biomediche per la Salute, Università degli Studi di Milano, Milano, Italy.
Fungal Genet Biol. 2016 Feb;87:22-9. doi: 10.1016/j.fgb.2016.01.003. Epub 2016 Jan 6.
Cryptococcus neoformans var. neoformans (serotype D) represents about 30% of the clinical isolates in Europe and is present less frequently in the other continents. It is the prevalent etiological agent in primary cutaneous cryptococcosis as well as in cryptococcal skin lesions of disseminated cryptococcosis. Very little is known about the genotypic diversity of this Cryptococcus subtype. The aim of this study was to investigate the genotypic diversity among a set of clinical and environmental C. neoformans var. neoformans isolates and to evaluate the relationship between genotypes, geographical origin and clinical manifestations. A total of 83 globally collected C. neoformans var. neoformans isolates from Italy, Germany, France, Belgium, Denmark, Greece, Turkey, Thailand, Japan, Colombia, and the USA, recovered from different sources (primary and secondary cutaneous cryptococcosis, disseminated cryptococcosis, the environment, and animals), were included in the study. All isolates were confirmed to belong to genotype VNIV by molecular typing and they were further investigated by MLST analysis. Maximum likelihood phylogenetic as well as network analysis strongly suggested the existence of a recombinant rather than a clonal population structure. Geographical origin and source of isolation were not correlated with a specific MLST genotype. The comparison with a set of outgroup C. neoformans var. grubii isolates provided clear evidence that the two varieties have different population structures.
新型隐球菌新生变种（血清型D）约占欧洲临床分离株的30%，在其他各大洲出现的频率较低。它是原发性皮肤隐球菌病以及播散性隐球菌病的皮肤隐球菌病变中的主要病原体。关于这种隐球菌亚型的基因型多样性知之甚少。本研究的目的是调查一组临床和环境来源的新型隐球菌新生变种分离株的基因型多样性，并评估基因型、地理来源和临床表现之间的关系。本研究纳入了总共83株从意大利、德国、法国、比利时、丹麦、希腊、土耳其、泰国、日本、哥伦比亚和美国全球收集的新型隐球菌新生变种分离株，这些分离株来自不同来源（原发性和继发性皮肤隐球菌病、播散性隐球菌病、环境和动物）。通过分子分型确认所有分离株均属于VNIV基因型，并通过多位点序列分型分析对其进行进一步研究。最大似然系统发育分析以及网络分析强烈表明存在重组而非克隆的群体结构。地理来源和分离源与特定的多位点序列分型基因型无关。与一组外群新型隐球菌格鲁比变种分离株的比较提供了明确证据，表明这两个变种具有不同的群体结构。
