Zhang Heng, Guo Mengqing, Wu Qiaona, Zhao Mengqiu, Li Ruiping, Deng Xiaomei, Xi Ruchun
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou, China.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.
Front Plant Sci. 2022 Aug 12;13:914652. doi: 10.3389/fpls.2022.914652. eCollection 2022.
is one of the main timber trees grown in China. However, severe shortage of natural seeds and the difficulty of explant regeneration has limited seedling supply. As such, there is a need for research on asexual multiplication of . This study established a rapid propagation technology system for genotypes, including explant treatment, proliferation, and rooting. HZ (a modified MS medium) supplemented with 4.4 μM BA and 0.5 μM IBA was found to be the optimal medium for shoot sprouting. The maximum proliferation coefficient and the number of effective shoots was obtained on HZ medium supplemented with 2.6 μM BA and 1.0 μM IBA, were 3.00 and 5.63, respectively. A rooting rate of 83.33% was achieved using half-strength HZ medium supplemented with 3.2 μM NAA. Adding vitamin C (80 mg⋅l) for 7 days in a dark environment reduced the browning rate, while increasing the proliferation rate. Additionally, through cytological observation, we established how and where adventitious roots occur. The survival rate of transplanted plantlets was > 90%. This is the first report of an regeneration technique that uses stem segments of mature as explants.
是中国主要的用材树种之一。然而，天然种子严重短缺以及外植体再生困难限制了苗木供应。因此，需要对[树种名称]的无性繁殖进行研究。本研究建立了[树种名称]基因型的快速繁殖技术体系，包括外植体处理（增殖和生根）。发现添加4.4 μM 苄氨基腺嘌呤（BA）和0.5 μM 吲哚丁酸（IBA）的HZ（改良的MS培养基）是芽萌发的最佳培养基。在添加2.6 μM BA和1.0 μM IBA的HZ培养基上获得了最大增殖系数和有效芽数，分别为3.00和5.63。使用添加3.2 μM 萘乙酸（NAA）的半强度HZ培养基，生根率达到83.33%。在黑暗环境中添加维生素C（80 mg·l）7天可降低褐变率，同时提高增殖率。此外，通过细胞学观察，我们确定了不定根产生的方式和位置。移栽苗的成活率>90%。这是首次报道利用成熟[树种名称]的茎段作为外植体的[树种名称]再生技术。
