Wang Hongman, Dong Zuqi, Chen Jianbing, Wang Meng, Ding Yuting, Xue Qingyun, Liu Wei, Niu Zhitao, Ding Xiaoyu
College of Life Sciences, Nanjing Normal University, Nanjing, China.
Jiangsu Provincial Engineering Research Center for Technical Industrialization for Dendrobium, Nanjing, China.
Front Plant Sci. 2022 Aug 10;13:979801. doi: 10.3389/fpls.2022.979801. eCollection 2022.
, an important orchid plant with great horticultural and medicinal values, frequently suffers from abiotic or biotic stresses in the wild, which may influence its well-growth. Heat shock proteins (s) play essential roles in the abiotic stress response of plants. However, they have not been systematically investigated in . Here, we identified 37 genes (s), 43 genes (s) and 4 genes (s) in genome. These genes were classified into 8, 4 and 2 subfamilies based on phylogenetic analysis and subcellular predication, respectively. Sequence analysis showed that the same subfamily members have relatively conserved gene structures and similar protein motifs. Moreover, we identified 33 pairs of paralogs containing 30 pairs of tandem duplicates and 3 pairs of segmental duplicates among these genes. There were 7 pairs in s under positive selection, which may have important functions in helping cells withstand extreme stress. Numerous gene promoter sequences contained stress and hormone response -elements, especially light and MeJA response elements. Under MeJA stress, s, s and s responded to varying degrees, among which extremely up-regulated, which may have a strong stress resistance. Therefore, these findings could provide useful information for evolutional and functional investigations of , and genes in .
作为一种具有重要园艺和药用价值的重要兰花植物,在野外经常遭受非生物或生物胁迫,这可能会影响其良好生长。热激蛋白在植物的非生物胁迫响应中发挥着重要作用。然而,它们尚未在[植物名称]中得到系统研究。在这里,我们在[植物名称]基因组中鉴定出37个[热激蛋白基因名称1]基因、43个[热激蛋白基因名称2]基因和4个[热激蛋白基因名称3]基因。基于系统发育分析和亚细胞预测,这些基因分别被分为8个、4个和2个亚家族。序列分析表明,同一亚家族成员具有相对保守的基因结构和相似的蛋白质基序。此外,我们在这些基因中鉴定出33对旁系同源基因,其中包含30对串联重复基因和3对片段重复基因。[热激蛋白基因名称1]中有7对处于正选择状态,这可能在帮助细胞抵御极端胁迫方面具有重要功能。许多基因启动子序列包含胁迫和激素响应元件,特别是光和茉莉酸甲酯响应元件。在茉莉酸甲酯胁迫下,[热激蛋白基因名称1]、[热激蛋白基因名称2]和[热激蛋白基因名称3]有不同程度的响应,其中[热激蛋白基因名称1]极度上调,这可能具有较强的抗胁迫能力。因此,这些发现可为[植物名称]中[热激蛋白基因名称1]、[热激蛋白基因名称2]和[热激蛋白基因名称3]基因的进化和功能研究提供有用信息。