野生二粒小麦（）和面包小麦（）基因家族的全基因组鉴定及系统发育关系
Genome-wide identification and phylogenetic relationships of the gene family of , wild emmer wheat () and bread wheat ().
作者信息
Lai Di-Li, Yan Jun, Fan Yu, Li Yao, Ruan Jing-Jun, Wang Jun-Zhen, Fan Yue, Cheng Xiao-Bin, Cheng Jian-Ping
机构信息
College of Agriculture, Guizhou University, Guiyang, 550025 People's Republic of China.
School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106 People's Republic of China.
出版信息
3 Biotech. 2021 Jun;11(6):301. doi: 10.1007/s13205-021-02639-5. Epub 2021 May 29.
UNLABELLED
Heat shock protein 70 () plays an important role in plant development. It is closely related to the physiological process of cell development and the response to abiotic and biological stress. However, the classification and evolution of genes in bread wheat, wild emmer wheat and are still unclear. Therefore, this study conducted a comprehensive bioinformatics analysis of gene in three species. Among these three species, 113, 79 and 36 genes were identified. They are divided into six subfamilies. Group vi-1 is different from . It may be the result of early evolutionary segregation. The number of exons in different subfamilies (from 1 to 13) was different, but the distribution patterns of exons / introns in the same subfamily were similar. The results of promoter region analysis showed that the cis-regulatory elements of and wild emmer wheat were different from those of wheat. In addition, CpG island proportion of wild emmer was higher than that of wheat, which may be the molecular basis of heat resistance of wild wheat relative to cultivated wheat. Further comprehensive analysis of chromosome location and repeat events of gene showed that whole-genome duplication and tandem duplication events contributed to the evolution and expansion of gene in wheat. The results of non-synonymous substitution and synonymous substitution analysis showed that genes of three species had undergone purification selection. The expression profile analysis showed that gene was highly expressed in the roots during the vegetative growth period. In addition, TaHsp70 gene was highly expressed under various stress. The identification, classification and evolution of in wheat and its relatives provided a basis for further research on its evolution and its molecular mechanism in response to stress.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-021-02639-5.
未标记
热休克蛋白70（Hsp70）在植物发育中起重要作用。它与细胞发育的生理过程以及对非生物和生物胁迫的响应密切相关。然而，面包小麦、野生二粒小麦和节节麦中Hsp70基因的分类和进化仍不清楚。因此，本研究对这三个物种中的Hsp70基因进行了全面的生物信息学分析。在这三个物种中，分别鉴定出113个、79个和36个Hsp70基因。它们被分为六个亚家族。第六组-1与其他组不同。这可能是早期进化分离的结果。不同亚家族中外显子的数量（从1到13）不同，但同一亚家族中外显子/内含子的分布模式相似。Hsp70启动子区域分析结果表明，节节麦和野生二粒小麦的顺式调控元件与普通小麦不同。此外，野生二粒小麦的CpG岛比例高于普通小麦，这可能是野生小麦相对于栽培小麦耐热性的分子基础。对Hsp70基因的染色体定位和重复事件的进一步综合分析表明，全基因组重复和串联重复事件促成了小麦中Hsp70基因的进化和扩张。非同义替换和同义替换分析结果表明，三个物种的Hsp70基因都经历了纯化选择。表达谱分析表明，Hsp70基因在营养生长期的根中高表达。此外，TaHsp70基因在各种胁迫下高表达。小麦及其近缘种中Hsp70的鉴定、分类和进化为进一步研究其进化及其响应胁迫的分子机制提供了依据。
补充信息
在线版本包含可在10.1007/s13205-021-02639-5获取的补充材料。
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