Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G DeGroote School of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
STAR Protoc. 2022 Aug 18;3(3):101628. doi: 10.1016/j.xpro.2022.101628. eCollection 2022 Sep 16.
Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).
人神经干细胞（hNSCs）是脑癌研究中的一种有价值的工具，因为它们被用作多种检测的正常对照，主要与毒性有关。在这里，我们提出了一个从人胚胎脑组织中安全、成功地获得和培养 hNSCs 的方案。我们描述了分离胚胎脑组织和培养 hNSCs 的步骤，然后是扩大 hNSCs 的过程。这些细胞可用于下游应用，包括 RNA-seq 和组学研究。有关此方案的使用和执行的完整详细信息，请参阅 Venugopal 等人（2012b）、Bakhshinyan 等人（2019）和 Venugopal 等人（2012a）。
