Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
STAR Protoc. 2024 Mar 15;5(1):102878. doi: 10.1016/j.xpro.2024.102878. Epub 2024 Feb 8.
Human pluripotent stem cell-derived neural progenitor cells (NPCs) are an essential tool for the study of brain development and developmental disorders such as autism. Here, we present a protocol to generate NPCs rapidly and reproducibly from human stem cells using dual-SMAD inhibition coupled with a brief pulse of mouse neurogenin-2 (Ngn2) overexpression. We detail the 48-h induction scheme deployed to produce these cells-termed stem cell-derived Ngn2-accelerated progenitor cells-followed by steps for expansion, purification, banking, and quality assessment. For complete details on the use and execution of this protocol, please refer to Wells et al..
人多能干细胞衍生的神经祖细胞（NPCs）是研究大脑发育和自闭症等发育障碍的重要工具。在这里，我们提出了一种使用双 SMAD 抑制结合短暂过表达小鼠神经基因 2（Ngn2）来快速且可重复地从人类干细胞中生成 NPCs 的方案。我们详细介绍了用于产生这些细胞的 48 小时诱导方案，这些细胞被称为干细胞衍生的 Ngn2 加速祖细胞，然后是扩展、纯化、储存和质量评估步骤。有关此方案的使用和执行的完整详细信息，请参阅 Wells 等人的研究。
