Chen Chuwen, Li Jie, Li Zhixiong
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, China.
College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China.
Arch Anim Breed. 2022 Aug 3;65(3):275-284. doi: 10.5194/aab-65-275-2022. eCollection 2022.
To investigate the effects of miR-499-5p on muscle metabolism in broiler chickens, eight broiler chicks were assigned to the control group and eight to the treatment group, and then we monitored the effects using metabolomics. Chicks were fed basal diets without or with miR-499-5p delivery. Gastrocnemius muscle samples were collected and analyzed by ultrahigh-performance liquid chromatography-tandem mass spectrometry. The results showed that miR-499-5p injection altered the concentrations of a variety of metabolites in the gastrocnemius muscle. Thereby, a total of 46 metabolites were identified at higher ( ) concentrations and 30 metabolites were identified at lower ( ) concentrations in the treatment group compared with the control group. These metabolites were primarily involved with the regulation of lipid and carbohydrate metabolism. Further metabolic pathway analysis revealed that fructose and mannose metabolism, galactose metabolism, inositol phosphate metabolism, and terpenoid backbone biosynthesis were the most critical pathway which may partially interpret the effects of miR-499-5p. To our knowledge, this research is the first report of metabolic signatures and related metabolic pathways in the skeletal muscle for miR-499-5p injection and provides new insight into the effect of miRNA on growth performance.
为研究miR-499-5p对肉鸡肌肉代谢的影响，将8只肉鸡雏鸡分配至对照组，8只分配至处理组，然后我们使用代谢组学监测其效果。雏鸡分别饲喂不含或含有miR-499-5p的基础日粮。采集腓肠肌样本并通过超高效液相色谱-串联质谱进行分析。结果显示，注射miR-499-5p改变了腓肠肌中多种代谢物的浓度。因此，与对照组相比，处理组中共有46种代谢物在较高（ ）浓度下被鉴定出来，30种代谢物在较低（ ）浓度下被鉴定出来。这些代谢物主要参与脂质和碳水化合物代谢的调节。进一步的代谢途径分析表明，果糖和甘露糖代谢、半乳糖代谢、肌醇磷酸代谢和萜类骨架生物合成是最关键的途径，这可能部分解释了miR-499-5p的作用。据我们所知，本研究是关于注射miR-499-5p后骨骼肌中代谢特征及相关代谢途径的首次报道，并为miRNA对生长性能的影响提供了新的见解。
