Tan Chengkeng, Selamat Jinap, Jambari Nuzul Noorahya, Sukor Rashidah, Murugesu Suganya, Khatib Alfi
Food Safety and Food Integrity (FOSFI), Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
National Public Health Laboratory, Ministry of Health Malaysia, Lot 1853, Kampung Melayu Sungai Buloh, Sungai Buloh 47000, Selangor, Malaysia.
Foods. 2021 Sep 14;10(9):2174. doi: 10.3390/foods10092174.
Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored broiler (Hubbard), broiler (Cobb), and spent laying hen (Dekalb) in pectoralis major and serum under commercial conditions using an untargeted metabolomics approach. Both pectoralis major and serum were analyzed using gas chromatography-mass spectrometry (GC-MS). The principal component analysis (PCA) results distinguished four different chicken breeds into three main groups for pectoralis major and serum. A total of 30 and 40 characteristic metabolites were identified for pectoralis major and serum, respectively. The four chicken breeds were characterized by the abundance of metabolites such as amino acids (L-glutamic acid, L-threonine, L-serine, L-leucine), organic acids (L-lactic acid, succinic acid, 3-hydroxybutyric acid), sugars (D-allose, D-glucose), sugar alcohols (myo-inositol), and fatty acids (linoleic acid). Our results suggest that an untargeted metabolomics approach using GC-MS and PCA could discriminate chicken breeds for pectoralis major and serum under commercial conditions. In this study, village chicken could only be distinguished from colored broiler (Hubbard) by serum samples.
在全球范围内，乡村鸡很受欢迎，被认为是一种价格较高的优质肉类。在当地市场上，可能会出现以高价出售其他品种鸡来进行食品欺诈的情况。本研究旨在使用非靶向代谢组学方法，在商业条件下区分当地乡村鸡与市场上其他可用的鸡品种，即有色肉鸡（哈伯德）、肉鸡（科布）和产蛋后期母鸡（迪卡布）的胸大肌和血清。使用气相色谱 - 质谱联用仪（GC-MS）对胸大肌和血清进行分析。主成分分析（PCA）结果将四种不同的鸡品种在胸大肌和血清方面分为三个主要组。分别为胸大肌和血清鉴定出30种和40种特征代谢物。这四种鸡品种的特征在于氨基酸（L-谷氨酸、L-苏氨酸、L-丝氨酸、L-亮氨酸）、有机酸（L-乳酸、琥珀酸、3-羟基丁酸）、糖类（D-阿洛糖、D-葡萄糖）、糖醇（肌醇）和脂肪酸（亚油酸）等代谢物的丰度。我们的结果表明，使用GC-MS和PCA的非靶向代谢组学方法可以在商业条件下区分鸡的品种。在本研究中，乡村鸡只能通过血清样本与有色肉鸡（哈伯德）区分开来。
