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High-throughput sequencing revealed low-efficacy genome editing using Cas9 RNPs electroporation and single-celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis.

作者信息

Chen Zhenfan, Zhu Jiayi, Chen Zixi, Du Ming, Yao Rao, Fu Wen, Lei Anping, Wang Jiangxin

机构信息

Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.

出版信息

Plant Biotechnol J. 2022 Nov;20(11):2048-2050. doi: 10.1111/pbi.13915. Epub 2022 Sep 15.

DOI:10.1111/pbi.13915
PMID:36036114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9616521/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/657a/11382902/9f0c926df0e5/PBI-20-2048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/657a/11382902/9f0c926df0e5/PBI-20-2048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/657a/11382902/9f0c926df0e5/PBI-20-2048-g001.jpg

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本文引用的文献

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A Synthetic Biology Perspective on the Bioengineering Tools for an Industrial Microalga: .从合成生物学角度看工业微藻的生物工程工具：
Front Bioeng Biotechnol. 2022 Apr 6;10:882391. doi: 10.3389/fbioe.2022.882391. eCollection 2022.
2
Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in .在. 中高效的基于 CRISPR 相关蛋白 9 核糖核蛋白的基因组编辑。
STAR Protoc. 2020 Jun 3;1(1):100023. doi: 10.1016/j.xpro.2020.100023. eCollection 2020 Jun 19.
3
Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins.
医疗保健和功能性食品中的微藻：β-葡聚糖的应用、药物递送创新与合成生物学
Front Pharmacol. 2025 Mar 4;16:1557298. doi: 10.3389/fphar.2025.1557298. eCollection 2025.
4
A chromosome-level genome assembly for the paramylon-producing microalga Euglena gracilis.为产藻朊小球藻的微藻小球藻构建染色体水平的基因组组装。
Sci Data. 2024 Jul 16;11(1):780. doi: 10.1038/s41597-024-03404-y.
5
Versatile biotechnological applications of Euglena gracilis.绿眼虫在生物技术上的多种应用。
World J Microbiol Biotechnol. 2023 Mar 24;39(5):133. doi: 10.1007/s11274-023-03585-5.
利用Cas9核糖核蛋白在工业微藻纤细裸藻中进行高效无转基因靶向诱变和单链寡脱氧核苷酸介导的精确敲入
Plant Biotechnol J. 2019 Nov;17(11):2032-2034. doi: 10.1111/pbi.13174. Epub 2019 Jun 11.
4
Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.在斑马鱼胚胎中显微注射信使核糖核酸（mRNA）和吗啉代反义寡核苷酸。
J Vis Exp. 2009 May 7(27):1113. doi: 10.3791/1113.
5
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Curr Opin Biotechnol. 2008 Oct;19(5):506-10. doi: 10.1016/j.copbio.2008.07.005. Epub 2008 Sep 3.
6
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