Adriaans B, Hay R J, Drasar B S, Robinson D C
Trans R Soc Trop Med Hyg. 1986;80(5):793-4. doi: 10.1016/0035-9203(86)90385-8.
The successful isolation of anaerobes largely depends on adequate transportation of the specimens after collection. We are investigating the role of anaerobes in the pathogenesis of tropical ulcer and have used Hungate tubes with pre-reduced peptone yeast broth and agar as a transportation system. The medium included 0.05% cysteine and 0.03% sodium formaldehyde sulphoxylate as an Eh buffer. Resazurin 1 microgram/ml seved as an Eh indicator. We found the tubes convenient to use and very reliable at maintaining anaerobiasis.
厌氧菌的成功分离在很大程度上取决于采集后标本的妥善运送。我们正在研究厌氧菌在热带溃疡发病机制中的作用，并使用装有预还原蛋白胨酵母肉汤和琼脂的亨盖特管作为运送系统。该培养基含有0.05%的半胱氨酸和0.03%的甲醛次硫酸氢钠作为氧化还原电位缓冲剂。1微克/毫升的刃天青用作氧化还原电位指示剂。我们发现这些管子使用方便，而且在维持无氧状态方面非常可靠。
