RNA-seq 分析揭示了碘酸钠诱导的大鼠视网膜变性模型中差异表达的炎症趋化因子。
RNA-seq analysis reveals differentially expressed inflammatory chemokines in a rat retinal degeneration model induced by sodium iodate.
机构信息
Shenzhen Eye Hospital, Shenzhen Key Laboratory of Ophthalmology, Affiliated Shenzhen Eye Hospital of Jinan University, Shenzhen, Guangdong, China.
The Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
出版信息
J Int Med Res. 2022 Aug;50(8):3000605221119376. doi: 10.1177/03000605221119376.
OBJECTIVE
Retinal degeneration (RD) is a group of serious blinding eye diseases characterized by photoreceptor cell apoptosis and progressive degeneration of retinal neurons. However, the underlying mechanism of its pathogenesis remains unclear.
METHODS
In this study, retinal tissues from sodium iodate (NaIO)-induced RD and control rats were collected for transcriptome analysis using RNA-sequencing (RNA-seq). Analysis of white blood cell-related parameters was conducted in patients with retinitis pigmentosa (RP) and age-related cataract (ARC) patients.
RESULTS
In total, 334 mRNAs, 77 long non-coding RNAs (lncRNAs), and 20 other RNA types were identified as differentially expressed in the retinas of NaIO-induced RD rats. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that differentially expressed mRNAs were mainly enriched in signaling pathways related to immune inflammation. Moreover, we found that the neutrophil-to-lymphocyte ratio was significantly higher in RP patients than in ARC patients.
CONCLUSION
Overall, this study suggests that multiple chemokines participating in systemic inflammation may contribute to RD pathogenesis.
目的
视网膜变性（RD）是一组以光感受器细胞凋亡和视网膜神经元进行性变性为特征的严重致盲眼病。然而，其发病机制的潜在机制仍不清楚。
方法
在这项研究中，使用 RNA 测序（RNA-seq）对碘酸钠（NaIO）诱导的 RD 和对照大鼠的视网膜组织进行了转录组分析。对视网膜色素变性（RP）和年龄相关性白内障（ARC）患者的白细胞相关参数进行了分析。
结果
共鉴定出 334 个 mRNA、77 个长非编码 RNA（lncRNA）和 20 种其他 RNA 类型在 NaIO 诱导的 RD 大鼠视网膜中表达差异。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，差异表达的 mRNAs 主要富集在与免疫炎症相关的信号通路中。此外，我们发现 RP 患者的中性粒细胞与淋巴细胞比值明显高于 ARC 患者。
结论
总的来说，这项研究表明，参与全身炎症的多种趋化因子可能有助于 RD 的发病机制。
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