Molecular Microbiology Lab, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam.
Arch Virol. 2022 Nov;167(11):2337-2346. doi: 10.1007/s00705-022-05580-x. Epub 2022 Aug 29.
The spike protein (S) of porcine epidemic diarrhea virus (PEDV), in particular, the C-terminal domain of the S1 subunit (S1-CTD), which contains the conserved CO-26K-equivalent (COE) region (aa 499-638), which is recognized by neutralizing antibodies, exhibits a high degree of genetic and antigenic diversity. We analyzed 61 PEDV S1-CTD sequences (630 nt), including 26 from samples collected from seven provinces in northern Vietnam from 2018 to 2019 and 35 other sequences, representing the G1a and 1b, G2a and 2b, and recombinant (G1c) genotypes and vaccines. The majority (73.1%) of the strains (19/26) belonged to subgroup G2b. In a phylogenetic analysis, seven strains were clustered into an independent, distinct subgenogroup named dsG with strong nodal support (98%), separate from both G1a and G1b as well as G2a, 2b, and G1c. Sequence analysis revealed distinct changes (513, 520, 527, 591, 669, and 691) in the COE and S1 regions that were only identified in these Vietnamese strains. This cluster is a new antigenic variant subgroup, and further studies are required to investigate the antigenicity of these variants. The results of this study demonstrated the continuous evolution in the S1 region of Vietnamese PEDV strains, which emphasizes the need for frequent updates of vaccines for effective protection.
猪流行性腹泻病毒（PEDV）的刺突蛋白（S），特别是 S1 亚基的 C 末端结构域（S1-CTD），其中包含保守的 CO-26K 等效区（COE）（aa 499-638），该区域被中和抗体识别，表现出高度的遗传和抗原多样性。我们分析了 61 个 PEDV S1-CTD 序列（630nt），包括 2018 年至 2019 年从越南北部七个省份采集的 26 个样本，以及代表 G1a 和 1b、G2a 和 2b 以及重组（G1c）基因型和疫苗的 35 个其他序列。大多数（73.1%）菌株（19/26）属于亚组 G2b。在系统发育分析中，有 7 个毒株聚类成一个独立的、独特的亚群，称为 dsG，具有强烈的节点支持（98%），与 G1a 和 G1b 以及 G2a、2b 和 G1c 分开。序列分析显示在 COE 和 S1 区有明显的变化（513、520、527、591、669 和 691），这些变化仅在这些越南毒株中发现。该聚类是一个新的抗原变异亚群，需要进一步研究这些变异株的抗原性。本研究结果表明越南 PEDV 株 S1 区的持续进化，这强调了为有效保护需要频繁更新疫苗。
