Callicoat P A, Hicks M W, Smith D M, Clark D A
Transfusion. 1987 Jul-Aug;27(4):332-4. doi: 10.1046/j.1537-2995.1987.27487264742.x.
Red cells depleted of leukocytes by the "spin, cool, and filter" (SCF) method are effective in preventing most febrile, nonhemolytic transfusion reactions (FNHTR). To determine whether red cell concentrates may be centrifuged in a blood center and filtered subsequently at an outlying facility, the authors examined how leukocyte removal was affected by the transport and storage of centrifuged red cells before microaggregate filtration (MAF). One hundred fourteen red cell units were each divided into two aliquots. After centrifugation, one aliquot from each unit was retained in the blood center, and the other was transported on a truck for 2 to 12 hours. Aliquots were stored for variable periods, after which the residual leukocytes were counted. Neither transportation nor storage significantly affected leukocyte removal by MAF. However, an unacceptable proportion of all SCF units failed to meet American Red Cross standards for leukocyte-poor blood and studies of factors influencing leukocyte depletion were undertaken. A relative centrifugal force of 6700 X g was needed to produce consistently acceptable units when blood was 8 to 15 days old. These results show that making SCF red cells is a practical, inexpensive way for blood centers to provide hospitals with products that prevent most FNHTR; however, each facility that prepares these products must perform quality control carefully.
采用“旋转、冷却和过滤”（SCF）方法去除白细胞的红细胞在预防大多数发热性非溶血性输血反应（FNHTR）方面是有效的。为了确定红细胞浓缩液是否可以在血液中心进行离心，然后在偏远机构进行过滤，作者研究了在微聚集体过滤（MAF）之前，离心后的红细胞的运输和储存对白细胞去除的影响。114个红细胞单位各分为两份。离心后，每个单位的一份留在血液中心，另一份用卡车运输2至12小时。各份储存不同时间，之后对残留白细胞进行计数。运输和储存均未显著影响MAF对白细胞的去除。然而，所有SCF单位中有不可接受比例的产品未达到美国红十字会对低白细胞血液的标准，因此开展了影响白细胞去除因素的研究。当血液保存8至15天时，需要6700×g的相对离心力才能始终生产出可接受的单位产品。这些结果表明，制备SCF红细胞是血液中心为医院提供预防大多数FNHTR产品的一种实用、廉价的方法；然而，每个制备这些产品的机构都必须认真进行质量控制。
