Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, Canada.
J Comput Biol. 2022 Nov;29(11):1229-1232. doi: 10.1089/cmb.2022.0251. Epub 2022 Aug 25.
UNIFAN is an unsupervised cell type annotation tool for single-cell RNA sequencing data (scRNA-seq). Given single-cell expression data as input, UNIFAN outputs cell clusters as well as annotations for each cluster. The clustering process utilizes information on pathways and biological processes and these are also used to annotate the resulting clusters. In this software article, we focus on how to install UNIFAN and on the main steps involved in using UNIFAN for cell type annotations.
UNIFAN 是一种用于单细胞 RNA 测序数据(scRNA-seq)的无监督细胞类型注释工具。给定单细胞表达数据作为输入,UNIFAN 输出细胞簇以及每个簇的注释。聚类过程利用了通路和生物过程的信息,这些信息也用于注释生成的簇。在本软件文章中,我们重点介绍如何安装 UNIFAN 以及使用 UNIFAN 进行细胞类型注释的主要步骤。