Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, No.268 Donghuan Road, Chengzhong District, Liuzhou City, 545006, Guangxi Zhuang Autonomous Region, China; Provine and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, 530004, Guangxi Zhuang Autonomous Region, China.
Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, No.268 Donghuan Road, Chengzhong District, Liuzhou City, 545006, Guangxi Zhuang Autonomous Region, China; Provine and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, 530004, Guangxi Zhuang Autonomous Region, China.
Anal Chim Acta. 2022 Sep 8;1225:340253. doi: 10.1016/j.aca.2022.340253. Epub 2022 Aug 14.
As a kind of acute cardiovascular disease, acute myocardial infarction (AMI) endangers human's life and fitness seriously. The detection of cardiac troponin I (cTnI), a biomarker of AMI, is the key to early medical prognosis and treatment. Surface-enhanced Raman spectroscopy is viewed to be an effective approach for detection of low-concentration substances, and aptamers are regarded to be effective fragments to recognize proteins specifically. In this work, a magnetic nanoparticle substrate coated with coral-like nano-silver was prepared, and the aptamer-modified substrate FeO@PEI/Ag NC-Apt was used as magnetic capture probe. Combined with BCA method and SERS detection technology, an Apt-SERS platform was successfully constructed and used for high sensitivity quantitative detection of protein. Taking cTnI as the target, the detection range of the proposed Apt-SERS platform was 0.001-100 ng mL, and the estimated detection of limit (LOD) was 0.23 pg mL. The recovery and relative standard deviations (RSD) of spiked experiment in human serum samples were 92-106% and 3.8-10.1%, respectively. The platform combining BCA method and SERS provides a high sensitivity and good reproducibility method for cTnI detection, which offers a new method for the specific detection and analysis of proteins.
作为一种急性心血管疾病，急性心肌梗死（AMI）严重威胁着人类的生命和健康。心肌肌钙蛋白 I（cTnI）作为 AMI 的生物标志物的检测，是早期医疗预后和治疗的关键。表面增强拉曼光谱被认为是检测低浓度物质的有效方法，适体被认为是特异性识别蛋白质的有效片段。在这项工作中，制备了一种涂覆珊瑚状纳米银的磁性纳米颗粒基底，并将适体修饰的基底 FeO@PEI/Ag NC-Apt 用作磁性捕获探针。结合 BCA 法和 SERS 检测技术，成功构建了适体增强拉曼光谱（Apt-SERS）平台，并用于蛋白质的高灵敏度定量检测。以 cTnI 为目标，所提出的 Apt-SERS 平台的检测范围为 0.001-100ng/mL，估计的检测限（LOD）为 0.23pg/mL。在人血清样品的加标实验中，回收率和相对标准偏差（RSD）分别为 92-106%和 3.8-10.1%。结合 BCA 法和 SERS 的平台为 cTnI 检测提供了一种高灵敏度和良好重现性的方法，为蛋白质的特异性检测和分析提供了一种新方法。
