Rezvan Amir, Sharifikolouei Elham, Lassnig Alice, Soprunyuk Viktor, Gammer Christoph, Spieckermann Florian, Schranz Wilfried, Najmi Ziba, Cochis Andrea, Scalia Alessandro Calogero, Rimondini Lia, Manfredi Marcello, Eckert Jürgen, Sarac Baran
Erich Schmid Institute of Materials Science, Austrian Academy of Sciences, A-8700, Leoben, Austria.
Department of Materials Science, Chair of Materials Physics, Montanuniversität Leoben, A-8700, Leoben, Austria.
Mater Today Bio. 2022 Aug 3;16:100378. doi: 10.1016/j.mtbio.2022.100378. eCollection 2022 Dec.
This paper envisions TiZrCuPd bulk metallic glass as an oral implant material and evaluates its antibacterial performance in the inhabitation of oral biofilm formation in comparison with the gold standard Ti-6Al-4V implant material. Metallic glasses are superior in terms of biocorrosion and have a reduced stress shielding effect compared with their crystalline counterparts. Dynamic mechanical and thermal expansion analyses on TiZrCuPd show that these materials can be thermomechanically shaped into implants. Static water contact angle measurement on samples' surface shows an increased surface wettability on the Ti-6Al-4V surface after 48 h incubation in the water while the contact angle remains constant for TiZrCuPd. Further, high-resolution transmission and scanning transmission electron microscopy analysis have revealed that TiZrCuPd interior is fully amorphous, while a 15 nm surface oxide is formed on its surface and assigned as copper oxide. Unlike titanium oxide formed on Ti-6Al-4V, copper oxide is hydrophobic, and its formation reduces surface wettability. Further surface analysis by X-ray photoelectron spectroscopy confirmed the presence of copper oxide on the surface. Metallic glasses cytocompatibility was first demonstrated towards human gingival fibroblasts, and then the antibacterial properties were verified towards the oral pathogen responsible for oral biofilm formation. After 24 h of direct infection, metallic glasses reported a >70% reduction of bacteria viability and the number of viable colonies was reduced by ∼8 times, as shown by the colony-forming unit count. Field emission scanning electron microscopy and fluorescent images confirmed the lower surface colonization of metallic glasses in comparison with controls. Finally, oral biofilm obtained from healthy volunteers was cultivated onto specimens' surface, and proteomics was applied to study the surface property impact on species composition within the oral plaque.
本文设想将TiZrCuPd块状金属玻璃作为口腔植入材料，并与金标准Ti-6Al-4V植入材料相比，评估其在抑制口腔生物膜形成方面的抗菌性能。金属玻璃在生物腐蚀方面表现优异，与晶体对应物相比，其应力屏蔽效应降低。对TiZrCuPd进行的动态力学和热膨胀分析表明，这些材料可以通过热机械加工成型为植入物。对样品表面进行静态水接触角测量表明，Ti-6Al-4V表面在水中孵育48小时后表面润湿性增加，而TiZrCuPd的接触角保持不变。此外，高分辨率透射和扫描透射电子显微镜分析表明，TiZrCuPd内部完全非晶态，而其表面形成了一层15纳米的表面氧化物，被确定为氧化铜。与Ti-6Al-4V上形成的氧化钛不同，氧化铜是疏水的，其形成降低了表面润湿性。通过X射线光电子能谱进行的进一步表面分析证实了表面存在氧化铜。首先证明了金属玻璃对人牙龈成纤维细胞具有细胞相容性，然后验证了其对导致口腔生物膜形成的口腔病原体的抗菌性能。直接感染24小时后，金属玻璃的细菌活力降低了70%以上，活菌菌落数量减少了约8倍，这通过菌落形成单位计数得到证明。场发射扫描电子显微镜和荧光图像证实，与对照相比，金属玻璃的表面定植较少。最后，将从健康志愿者获得的口腔生物膜培养在标本表面，并应用蛋白质组学研究表面性质对口腔菌斑内物种组成的影响。
