Cochis Andrea, Azzimonti Barbara, Chiesa Roberto, Rimondini Lia, Gasik Michael
Department of Health Sciences, Università del Piemonte Orientale (UPO), Via Solaroli 17, 28100 Novara, Italy.
Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali INSTM, Via Giusti 9, 50121 Firenze, Italy.
ACS Biomater Sci Eng. 2019 Jun 10;5(6):2815-2820. doi: 10.1021/acsbiomaterials.9b00147. Epub 2019 May 10.
Orthopedic metallic devices are often related with devasting complications due to acute prosthetic joint infections. Gallium (Ga) antibacterial activity has been demonstrated by the evidence that Ga in solution ionizes in a Ga trivalent form that replace Fe thus arresting metabolism. However, it is not clear whether such effect is restricted only to Ga release laps. Accordingly, here we investigated Ga addition into titanium alloys using metallurgical methods, thus realizing intermetallides of a very high stability that contain Ga in the range of 1, 2, 20, and 23% wt. ICP-OES analysis confirmed that Ga ions were not released from the specimens regardless of the Ga amount. These alloys ensured long-lasting Ga effect toward multidrug resistant , whose metabolic activity was reduced of >80% in comparison with controls. Finally, specimens cytocompatibility was confirmed by direct and indirect contact evaluations with mature osteoblasts and preosteoblasts progenitor cells.
由于急性人工关节感染，骨科金属器械常常与严重并发症相关。有证据表明镓（Ga）具有抗菌活性，即溶液中的Ga以三价形式离子化，取代Fe从而抑制新陈代谢。然而，尚不清楚这种效应是否仅局限于Ga的释放阶段。因此，我们在此使用冶金方法研究了在钛合金中添加Ga的情况，从而实现了具有非常高稳定性的金属间化合物，其含Ga量分别为1%、2%、20%和23%（重量）。电感耦合等离子体发射光谱（ICP - OES）分析证实，无论Ga含量如何，样品均未释放出Ga离子。这些合金对多重耐药菌具有持久的Ga效应，与对照相比，其代谢活性降低了80%以上。最后，通过与成熟成骨细胞和前成骨细胞祖细胞的直接和间接接触评估，证实了样品的细胞相容性。
