School of Chemical Science and Technology & Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan University, Kunming, 650091, P. R. China.
Chem Commun (Camb). 2022 Sep 15;58(74):10404-10407. doi: 10.1039/d2cc03482d.
The pyrazoline fluorophore, generated by photoinduced tetrazole-ene cycloaddition, shows faint fluorescence in protic solvents. To suppress this fluorescence-quenching, we rationally designed a series of substituted diaryl tetrazoles at the N-side phenyl ring to produce a tetrazole-ene based photoactivatable fluorophore. Spectroscopic and cellular imaging studies demonstrated that the version of the fluorophore with a bis(trifluoromethyl)benzene substituent exhibited significantly enhanced brightness and photostability.
吡唑啉荧光团通过光诱导三唑-烯环加成反应生成，在质子溶剂中显示微弱的荧光。为了抑制这种荧光猝灭，我们在 N-侧苯环上合理设计了一系列取代的二芳基四唑，以生成基于四唑-烯的光活化荧光团。光谱和细胞成像研究表明，带有双（三氟甲基）苯取代基的荧光团版本表现出显著增强的亮度和光稳定性。
