Confer A W, Simons K R, Barrie M T, Clinkenbeard K D
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
Vet Res Commun. 1990;14(3):175-80. doi: 10.1007/BF00347735.
Pasteurella haemolytica leukotoxin is a pore-forming cytolysin which acts as a virulence factor in pasteurellosis of domestic ruminants. Leukocytes from cattle, sheep and goats are susceptible to leukotoxin-induced lysis; however, leukocytes from non-ruminant species so far tested are resistant to leukotoxin-induced lysis. Neutrophils obtained from three white-tailed deer, four Saiga antelope, an Addra gazelle, a Grant's gazelle and a Sable antelope were tested for susceptibility to the lytic effects of P. haemolytica leukotoxin using lactate dehydrogenase release. Results were compared to those obtained using neutrophils from a steer and cultured bovine lymphoma cells. Neutrophils obtained from all these ruminants, except the Addra gazelle, were susceptible to P. haemolytica leukotoxin. Individual variation among the Saiga and the deer did not appear to be due to the percentages of neutrophils or the percentage of contaminating erythrocytes in the cell preparations.
溶血巴氏杆菌白细胞毒素是一种形成孔道的细胞溶素，在家养反刍动物巴氏杆菌病中作为一种毒力因子发挥作用。来自牛、绵羊和山羊的白细胞易受白细胞毒素诱导的裂解作用影响；然而，迄今为止所测试的非反刍动物物种的白细胞对白细胞毒素诱导的裂解作用具有抗性。使用乳酸脱氢酶释放法，对从三只白尾鹿、四只高鼻羚羊、一只阿拉伯瞪羚、一只格兰特瞪羚和一只貂羚获取的中性粒细胞进行了溶血巴氏杆菌白细胞毒素裂解作用敏感性测试。将结果与使用来自一头公牛的中性粒细胞和培养的牛淋巴瘤细胞所获得的结果进行比较。除阿拉伯瞪羚外，从所有这些反刍动物获取的中性粒细胞对溶血巴氏杆菌白细胞毒素敏感。高鼻羚羊和鹿之间的个体差异似乎并非由于细胞制剂中中性粒细胞的百分比或污染红细胞的百分比所致。
